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2htn

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(New page: 200px<br /><applet load="2htn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2htn, resolution 2.50&Aring;" /> '''E. coli bacterioferr...)
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[[Image:2htn.gif|left|200px]]<br /><applet load="2htn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2htn, resolution 2.50&Aring;" />
 
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'''E. coli bacterioferritin in its as-isolated form'''<br />
 
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==Overview==
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==E. coli bacterioferritin in its as-isolated form==
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Escherichia coli bacterioferritin was serendipitously crystallized in a, novel cubic crystal form and its structure could be determined to 2.5 A, resolution despite a high degree of merohedral twinning. This is the first, report of crystallographic data on 'as-isolated' E. coli bacterioferritin., The ferroxidase active site contains positive difference density, consistent with two metal ions that had co-purified with the protein., X-ray fluorescence studies suggest that the metal composition is different, from that of previous structures and is a mix of zinc and native iron, ions. The ferroxidase-centre configuration displays a similar flexibility, as previously noted for other bacterioferritins.
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<StructureSection load='2htn' size='340' side='right'caption='[[2htn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2htn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21 Escherichia coli BL21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HTN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2htn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2htn OCA], [https://pdbe.org/2htn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2htn RCSB], [https://www.ebi.ac.uk/pdbsum/2htn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2htn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2htn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2htn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Escherichia coli bacterioferritin was serendipitously crystallized in a novel cubic crystal form and its structure could be determined to 2.5 A resolution despite a high degree of merohedral twinning. This is the first report of crystallographic data on 'as-isolated' E. coli bacterioferritin. The ferroxidase active site contains positive difference density consistent with two metal ions that had co-purified with the protein. X-ray fluorescence studies suggest that the metal composition is different from that of previous structures and is a mix of zinc and native iron ions. The ferroxidase-centre configuration displays a similar flexibility as previously noted for other bacterioferritins.
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==About this Structure==
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Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form.,van Eerde A, Wolterink-van Loo S, van der Oost J, Dijkstra BW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1061-6. Epub 2006 Oct 25. PMID:17077480<ref>PMID:17077480</ref>
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2HTN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FE and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HTN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form., van Eerde A, Wolterink-van Loo S, van der Oost J, Dijkstra BW, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1061-6. Epub 2006 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17077480 17077480]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2htn" style="background-color:#fffaf0;"></div>
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[[Category: Ferroxidase]]
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[[Category: Single protein]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Eerde, A.van.]]
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[[Category: Loo, S.Wolterink-Van.]]
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[[Category: Oost, J.Van.Der.]]
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[[Category: FE]]
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[[Category: HEM]]
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[[Category: ferroxidase centre]]
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[[Category: four-helix bundle]]
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[[Category: haem]]
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[[Category: iron binding site]]
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[[Category: protein shell]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:58:32 2007''
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==See Also==
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli BL21]]
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[[Category: Large Structures]]
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[[Category: Dijkstra BW]]
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[[Category: Van Der Oost J]]
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[[Category: Wolterink-Van Loo S]]
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[[Category: Van Eerde A]]

Current revision

E. coli bacterioferritin in its as-isolated form

PDB ID 2htn

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