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2i6z

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[[Image:2i6z.jpg|left|200px]]<br /><applet load="2i6z" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2i6z, resolution 1.90&Aring;" />
 
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'''X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme'''<br />
 
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==Overview==
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==X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme==
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The interactions of cisplatin and its analogues, transplatin, carboplatin, and oxaliplatin, with hen egg white lysozyme were analysed through ESI, mass spectrometry, and the resulting metallodrug-protein adducts, identified; the X-ray crystal structure of the cisplatin lysozyme, derivative, solved at 1.9 A resolution, reveals selective platination of, imidazole Nepsilon of His15.
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<StructureSection load='2i6z' size='340' side='right'caption='[[2i6z]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2i6z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPT:CISPLATIN'>CPT</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6z OCA], [https://pdbe.org/2i6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6z RCSB], [https://www.ebi.ac.uk/pdbsum/2i6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i6z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The interactions of cisplatin and its analogues, transplatin, carboplatin and oxaliplatin, with hen egg white lysozyme were analysed through ESI mass spectrometry, and the resulting metallodrug-protein adducts identified; the X-ray crystal structure of the cisplatin lysozyme derivative, solved at 1.9 A resolution, reveals selective platination of imidazole Nepsilon of His15.
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==About this Structure==
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ESI mass spectrometry and X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme.,Casini A, Mastrobuoni G, Temperini C, Gabbiani C, Francese S, Moneti G, Supuran CT, Scozzafava A, Messori L Chem Commun (Camb). 2007 Jan 14;(2):156-8. Epub 2006 Oct 26. PMID:17180231<ref>PMID:17180231</ref>
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2I6Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CPT:'>CPT</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=DMS:'>DMS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Sites: <scene name='pdbsite=AC1:Cpt+Binding+Site+For+Residue+A+300'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+A+500'>AC2</scene> and <scene name='pdbsite=AC3:Dms+Binding+Site+For+Residue+A+400'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Z OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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ESI mass spectrometry and X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme., Casini A, Mastrobuoni G, Temperini C, Gabbiani C, Francese S, Moneti G, Supuran CT, Scozzafava A, Messori L, Chem Commun (Camb). 2007 Jan 14;(2):156-8. Epub 2006 Oct 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17180231 17180231]
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</div>
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[[Category: Gallus gallus]]
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<div class="pdbe-citations 2i6z" style="background-color:#fffaf0;"></div>
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[[Category: Lysozyme]]
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[[Category: Single protein]]
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[[Category: Casini, A.]]
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[[Category: Messori, L.]]
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[[Category: Temperini, C.]]
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[[Category: CL]]
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[[Category: CPT]]
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[[Category: DMS]]
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[[Category: anticancer drugs]]
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[[Category: cisplatin-lysozyme adduct]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:13:01 2008''
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==See Also==
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Gallus gallus]]
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[[Category: Large Structures]]
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[[Category: Casini A]]
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[[Category: Messori L]]
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[[Category: Temperini C]]

Current revision

X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme

PDB ID 2i6z

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