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2o8v

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[[Image:2o8v.png|left|200px]]
 
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{{STRUCTURE_2o8v| PDB=2o8v | SCENE= }}
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==PAPS reductase in a covalent complex with thioredoxin C35A==
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<StructureSection load='2o8v' size='340' side='right'caption='[[2o8v]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2o8v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O8V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o8v OCA], [https://pdbe.org/2o8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o8v RCSB], [https://www.ebi.ac.uk/pdbsum/2o8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o8v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSH_ECOLI CYSH_ECOLI] Reduction of activated sulfate into sulfite.[HAMAP-Rule:MF_00063]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o8/2o8v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o8v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of Escherichia coli 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase in complex with E. coli thioredoxin 1 (Trx1) has been determined to 3.0 A resolution. The two proteins are covalently linked via a mixed disulfide that forms during nucleophilic attack of Trx's N-terminal cysteine on the Sgamma atom of the PAPS reductase S-sulfocysteine (E-Cys-Sgamma-SO3-), a central intermediate in the catalytic cycle. For the first time in a crystal structure, residues 235-244 in the PAPS reductase C-terminus are observed, depicting an array of interprotein salt bridges between Trx and the strictly conserved glutathione-like sequence, Glu238Cys239Gly240Leu241His242. The structure also reveals a Trx-binding surface adjacent to the active site cleft and regions of PAPS reductase associated with conformational change. Interaction at this site strategically positions Trx to bind the S-sulfated C-terminus and addresses the mechanism for requisite structural rearrangement of this domain. An apparent sulfite-binding pocket at the protein-protein interface explicitly orients the S-sulfocysteine Sgamma atom for nucleophilic attack in a subsequent step. Taken together, the structure of PAPS reductase in complex with Trx highlights the large structural rearrangement required to accomplish sulfonucleotide reduction and suggests a role for Trx in catalysis beyond the paradigm of disulfide reduction.
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===PAPS reductase in a covalent complex with thioredoxin C35A===
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3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle.,Chartron J, Shiau C, Stout CD, Carroll KS Biochemistry. 2007 Apr 3;46(13):3942-51. Epub 2007 Mar 13. PMID:17352498<ref>PMID:17352498</ref>
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{{ABSTRACT_PUBMED_17352498}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2o8v" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2o8v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O8V OCA].
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017352498</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Carroll, K S.]]
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[[Category: Large Structures]]
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[[Category: Chartron, J.]]
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[[Category: Carroll KS]]
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[[Category: Shiau, C.]]
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[[Category: Chartron J]]
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[[Category: Stout, C D.]]
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[[Category: Shiau C]]
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[[Category: Disulfide crosslinked complex]]
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[[Category: Stout CD]]
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[[Category: Oxidoreductase]]
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Current revision

PAPS reductase in a covalent complex with thioredoxin C35A

PDB ID 2o8v

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