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2o9z

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[[Image:2o9z.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the Apo form==
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|PDB= 2o9z |SIZE=350|CAPTION= <scene name='initialview01'>2o9z</scene>, resolution 2.494&Aring;
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<StructureSection load='2o9z' size='340' side='right'caption='[[2o9z]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+531'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[2o9z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O9Z FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.494&#8491;</td></tr>
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|GENE= rbmJ, rebH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o9z OCA], [https://pdbe.org/2o9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o9z RCSB], [https://www.ebi.ac.uk/pdbsum/2o9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o9z ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2oa1|2OA1]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o9z OCA], [http://www.ebi.ac.uk/pdbsum/2o9z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o9z RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/REBH_LENAE REBH_LENAE] Involved in the biosynthesis of the indolocarbazole antitumor agent rebeccamycin. Catalyzes the chlorination of tryptophan (Trp) at C7 position to yield 7-chlorotryptophan. The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:11983340</ref> <ref>PMID:15743914</ref> <ref>PMID:17260957</ref>
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== Evolutionary Conservation ==
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'''Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the Apo form'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/2o9z_consurf.spt"</scriptWhenChecked>
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2O9Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9Z OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The structure of flavin-dependent tryptophan 7-halogenase RebH., Bitto E, Huang Y, Bingman CA, Singh S, Thorson JS, Phillips GN Jr, Proteins. 2008 Jan 1;70(1):289-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17876823 17876823]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o9z ConSurf].
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[[Category: Lechevalieria aerocolonigenes]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Bingman, C A.]]
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<references/>
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[[Category: Bitto, E.]]
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__TOC__
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[[Category: Jr., G N.Phillips.]]
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</StructureSection>
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[[Category: biosynthetic protein]]
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[[Category: Large Structures]]
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[[Category: flavin]]
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[[Category: Lentzea aerocolonigenes]]
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[[Category: flavoprotein]]
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[[Category: Bingman CA]]
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[[Category: halogenase]]
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[[Category: Bitto E]]
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[[Category: rebeccamycin]]
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[[Category: Phillips Jr GN]]
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[[Category: rebh]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:14:51 2008''
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Current revision

Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the Apo form

PDB ID 2o9z

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