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2onh

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[[Image:2onh.gif|left|200px]]<br /><applet load="2onh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2onh, resolution 2.7&Aring;" />
 
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'''Crystal Structure of of limonene synthase with 2-fluorolinalyl diphosphate(FLPP)'''<br />
 
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==Overview==
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==Crystal Structure of of limonene synthase with 2-fluorolinalyl diphosphate(FLPP)==
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The crystal structure of (4S)-limonene synthase from Mentha spic ata, a, metal ion-dependent monoterpene cyclase that catalyzes the coupled, isomerization and cyclization of geranyl diphosphate, is reported at, 2.7-A; resolution in two forms liganded to the substrate and intermediate, analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain, interactions in the homodimer and for changes in diphosphate-metal ion, coordination and substrate binding conformation in the course of the, multistep reaction.
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<StructureSection load='2onh' size='340' side='right'caption='[[2onh]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2onh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mentha_spicata Mentha spicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ONH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=F3P:(1S)-1-[(1S)-1-FLUOROETHYL]-1,5-DIMETHYLHEXYL+TRIHYDROGEN+DIPHOSPHATE'>F3P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2onh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2onh OCA], [https://pdbe.org/2onh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2onh RCSB], [https://www.ebi.ac.uk/pdbsum/2onh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2onh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q40322_MENSP Q40322_MENSP]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/2onh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2onh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction.
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==About this Structure==
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Structure of limonene synthase, a simple model for terpenoid cyclase catalysis.,Hyatt DC, Youn B, Zhao Y, Santhamma B, Coates RM, Croteau RB, Kang C Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5360-5. Epub 2007 Mar 19. PMID:17372193<ref>PMID:17372193</ref>
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2ONH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mentha_spicata Mentha spicata] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=F3P:'>F3P</scene> and <scene name='pdbligand=BTB:'>BTB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Mn+Binding+Site+For+Residue+A+601'>AC1</scene>, <scene name='pdbsite=AC2:Mn+Binding+Site+For+Residue+A+602'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Residue+A+603'>AC3</scene>, <scene name='pdbsite=AC4:Mn+Binding+Site+For+Residue+B+1601'>AC4</scene>, <scene name='pdbsite=AC5:Mn+Binding+Site+For+Residue+B+1602'>AC5</scene>, <scene name='pdbsite=AC6:Mn+Binding+Site+For+Residue+B+1603'>AC6</scene>, <scene name='pdbsite=AC7:F3p+Binding+Site+For+Residue+A+600'>AC7</scene>, <scene name='pdbsite=AC8:Btb+Binding+Site+For+Residue+A+604'>AC8</scene> and <scene name='pdbsite=AC9:F3p+Binding+Site+For+Residue+B+1600'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of limonene synthase, a simple model for terpenoid cyclase catalysis., Hyatt DC, Youn B, Zhao Y, Santhamma B, Coates RM, Croteau RB, Kang C, Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5360-5. Epub 2007 Mar 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17372193 17372193]
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</div>
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<div class="pdbe-citations 2onh" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mentha spicata]]
[[Category: Mentha spicata]]
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[[Category: Single protein]]
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[[Category: Croteau R]]
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[[Category: Croteau, R.]]
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[[Category: Hyatt DC]]
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[[Category: Hyatt, D.C.]]
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[[Category: Kang C]]
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[[Category: Kang, C.]]
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[[Category: Youn B]]
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[[Category: Youn, B.]]
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[[Category: BTB]]
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[[Category: F3P]]
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[[Category: MN]]
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[[Category: 2 fluorogeranyl diphosphate]]
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[[Category: 2-fluorolinalyl diphosphate]]
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[[Category: crystal structure]]
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[[Category: geranyl diphosphate]]
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[[Category: linalyl diphosphate]]
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[[Category: lyase]]
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[[Category: monoterpene cyclase]]
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[[Category: monoterpene synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:13:12 2008''
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Current revision

Crystal Structure of of limonene synthase with 2-fluorolinalyl diphosphate(FLPP)

PDB ID 2onh

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