2owc

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{{Seed}}
 
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[[Image:2owc.png|left|200px]]
 
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==Structure of a covalent intermediate in Thermus thermophilus amylomaltase==
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The line below this paragraph, containing "STRUCTURE_2owc", creates the "Structure Box" on the page.
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<StructureSection load='2owc' size='340' side='right'caption='[[2owc]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2owc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OWC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=PRD_900110:acarbose-derived+trisaccharide'>PRD_900110</scene>, <scene name='pdbligand=SUI:(3-AMINO-2,5-DIOXO-1-PYRROLIDINYL)ACETIC+ACID'>SUI</scene></td></tr>
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{{STRUCTURE_2owc| PDB=2owc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2owc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2owc OCA], [https://pdbe.org/2owc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2owc RCSB], [https://www.ebi.ac.uk/pdbsum/2owc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2owc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MALQ_THETH MALQ_THETH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/2owc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2owc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent glycosyl enzyme intermediate and a covalent intermediate in complex with an analogue of a co-substrate and show how the structures of both enzyme and substrate respond to the changes required by the catalytic cycle as it proceeds. Notably, the catalytic nucleophile changes conformation dramatically during the reaction. Also, Gln-256 on the 250s loop is involved in orienting the substrate in the +1 site. The absence of a suitable base in the covalent intermediate structure explains the low hydrolysis activity.
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===Structure of a covalent intermediate in Thermus thermophilus amylomaltase===
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Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase.,Barends TR, Bultema JB, Kaper T, van der Maarel MJ, Dijkhuizen L, Dijkstra BW J Biol Chem. 2007 Jun 8;282(23):17242-9. Epub 2007 Apr 9. PMID:17420245<ref>PMID:17420245</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17420245}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2owc" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17420245 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17420245}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2OWC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWC OCA].
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==Reference==
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Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase., Barends TR, Bultema JB, Kaper T, van der Maarel MJ, Dijkhuizen L, Dijkstra BW, J Biol Chem. 2007 Jun 8;282(23):17242-9. Epub 2007 Apr 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17420245 17420245]
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[[Category: 4-alpha-glucanotransferase]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Barends, T R.M.]]
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[[Category: Barends TRM]]
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[[Category: Bultema, J B.]]
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[[Category: Bultema JB]]
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[[Category: Dijkhuizen, L.]]
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[[Category: Dijkhuizen L]]
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[[Category: Dijkstra, B W.]]
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[[Category: Dijkstra BW]]
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[[Category: Kaper, T.]]
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[[Category: Kaper T]]
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[[Category: Maarel, M J.E C.van der.]]
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[[Category: Van der Maarel MJEC]]
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[[Category: Alpha-amylase]]
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[[Category: Amylomaltase]]
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[[Category: Beta-alpha-barrel]]
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[[Category: Glycosyl-enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:04:56 2008''
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Current revision

Structure of a covalent intermediate in Thermus thermophilus amylomaltase

PDB ID 2owc

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