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2oz6

From Proteopedia

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[[Image:2oz6.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP==
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|PDB= 2oz6 |SIZE=350|CAPTION= <scene name='initialview01'>2oz6</scene>, resolution 2.800&Aring;
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<StructureSection load='2oz6' size='340' side='right'caption='[[2oz6]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Cmp+Binding+Site+For+Residue+A+302'>AC1</scene> and <scene name='pdbsite=AC2:Cmp+Binding+Site+For+Residue+A+301'>AC2</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CMP:ADENOSINE-3&#39;,5&#39;-CYCLIC-MONOPHOSPHATE'>CMP</scene>
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<table><tr><td colspan='2'>[[2oz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OZ6 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE= vfr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oz6 OCA], [https://pdbe.org/2oz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2oz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oz6 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oz6 OCA], [http://www.ebi.ac.uk/pdbsum/2oz6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oz6 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/VFR_PSEAE VFR_PSEAE] Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/2oz6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oz6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Virulence Factor Regulator (Vfr) enhances Pseudomonas aeruginosa pathogenicity through its role as a global transcriptional regulator. The crystal structure of Vfr shows it is a winged-helix DNA-binding protein like its homologue cAMP Receptor Protein (CRP). In addition to an expected primary cAMP-binding site, a second ligand-binding site is nestled between the N-terminal domain and the C-terminal helix-turn-helix domain. Unlike CRP, Vfr is a symmetric dimer in the absence of DNA. Removal of seven disordered N-terminal residues of Vfr prevents growth of P. aeruginosa.
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'''Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP'''
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Crystal Structure of Pseudomonas aeruginosa Virulence Factor Regulator.,Cordes TJ, Worzalla GA, Ginster AM, Forest KT J Bacteriol. 2011 Jun 10. PMID:21665969<ref>PMID:21665969</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2OZ6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OZ6 OCA].
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<div class="pdbe-citations 2oz6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Bright AR]]
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[[Category: Bright, A R.]]
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[[Category: Cordes TJ]]
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[[Category: Cordes, T J.]]
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[[Category: Forest KT]]
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[[Category: Forest, K T.]]
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[[Category: camp receptor protein]]
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[[Category: camp-binding protein]]
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[[Category: dna binding protein]]
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[[Category: dna-binding protein]]
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[[Category: helix-turn-helix]]
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[[Category: nucleotide binding protein]]
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[[Category: transcription factor]]
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[[Category: winged helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:25:25 2008''
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Current revision

Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP

PDB ID 2oz6

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