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2plb

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(New page: 200px<br /><applet load="2plb" size="350" color="white" frame="true" align="right" spinBox="true" caption="2plb, resolution 1.60&Aring;" /> '''D(GTATACC) under hyd...)
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[[Image:2plb.gif|left|200px]]<br /><applet load="2plb" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2plb, resolution 1.60&Aring;" />
 
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'''D(GTATACC) under hydrostatic pressure of 1.39 GPa'''<br />
 
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==Overview==
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==D(GTATACC) under hydrostatic pressure of 1.39 GPa==
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The behaviour of the d(GGTATACC) oligonucleotide has been investigated by, X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa, ( approximately 20 000 atm). Four 3D-structures of the A-DNA form (at, ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A, resolution. In addition to the diffraction pattern of the A-form, the, broad meridional streaks previously explained by occluded B-DNA octamers, within the channels of the crystalline A-form matrix were observed up to, at least 2 GPa. This work highlights an important property of nucleic, acids, their capability to withstand very high pressures, while keeping in, such conditions a nearly invariant geometry of base pairs that store and, carry genetic information. The double-helix base-paired architecture, behaves as a molecular spring, which makes it especially adapted to very, harsh conditions. These features may have contributed to the emergence of, a RNA World at prebiotic stage.
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<StructureSection load='2plb' size='340' side='right'caption='[[2plb]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2plb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PLB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2plb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2plb OCA], [https://pdbe.org/2plb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2plb RCSB], [https://www.ebi.ac.uk/pdbsum/2plb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2plb ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.
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==About this Structure==
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Adaptation of the base-paired double-helix molecular architecture to extreme pressure.,Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:17617642<ref>PMID:17617642</ref>
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2PLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Spm Binding Site For Residue B 20'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLB OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007 Jul 7;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17617642 17617642]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2plb" style="background-color:#fffaf0;"></div>
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[[Category: Fourme, R.]]
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== References ==
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[[Category: Girard, E.]]
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<references/>
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[[Category: Kahn, R.]]
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__TOC__
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[[Category: Prange, T.]]
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</StructureSection>
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[[Category: SPM]]
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[[Category: Large Structures]]
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[[Category: crystal structure]]
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[[Category: Fourme R]]
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[[Category: dna]]
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[[Category: Girard E]]
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[[Category: high-pressure]]
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[[Category: Kahn R]]
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[[Category: Prange T]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:42:52 2008''
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Current revision

D(GTATACC) under hydrostatic pressure of 1.39 GPa

PDB ID 2plb

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