1mjd
From Proteopedia
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'''Structure of N-terminal domain of human doublecortin''' | '''Structure of N-terminal domain of human doublecortin''' | ||
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[[Category: Otlewski, J.]] | [[Category: Otlewski, J.]] | ||
[[Category: Walsh, C A.]] | [[Category: Walsh, C A.]] | ||
| - | [[Category: | + | [[Category: Dcx domain]] |
| - | [[Category: | + | [[Category: Microtubule associated protein]] |
| - | [[Category: | + | [[Category: Ubiquitin-like fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:10:47 2008'' | |
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Revision as of 22:10, 2 May 2008
Structure of N-terminal domain of human doublecortin
Contents |
Overview
The doublecortin-like domains (DCX), which typically occur in tandem, are novel microtubule-binding modules. DCX tandems are found in doublecortin, a 360-residue protein expressed in migrating neurons; the doublecortin-like kinase (DCLK); the product of the RP1 gene that is responsible for a form of inherited blindness; and several other proteins. Mutations in the gene encoding doublecortin cause lissencephaly in males and the 'double-cortex syndrome' in females. We here report a solution structure of the N-terminal DCX domain of human doublecortin and a 1.5 A resolution crystal structure of the equivalent domain from human DCLK. Both show a stable, ubiquitin-like tertiary fold with distinct structural similarities to GTPase-binding domains. We also show that the C-terminal DCX domains of both proteins are only partially folded. In functional assays, the N-terminal DCX domain of doublecortin binds only to assembled microtubules, whereas the C-terminal domain binds to both microtubules and unpolymerized tubulin.
Disease
Known disease associated with this structure: Lissencephaly, X-linked OMIM:[300121], Subcortical laminal heteropia, X-linked OMIM:[300121]
About this Structure
1MJD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The DCX-domain tandems of doublecortin and doublecortin-like kinase., Kim MH, Cierpicki T, Derewenda U, Krowarsch D, Feng Y, Devedjiev Y, Dauter Z, Walsh CA, Otlewski J, Bushweller JH, Derewenda ZS, Nat Struct Biol. 2003 May;10(5):324-33. PMID:12692530 Page seeded by OCA on Sat May 3 01:10:47 2008
