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3btv

From Proteopedia

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Current revision

Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S0)-[G301R]

Structural highlights

3btv is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GAL80_YEAST This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

NADP regulates the yeast GAL induction system.,Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L Science. 2008 Feb 22;319(5866):1090-2. PMID:18292341^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L. NADP regulates the yeast GAL induction system. Science. 2008 Feb 22;319(5866):1090-2. PMID:18292341 doi:http://dx.doi.org/319/5866/1090

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3btv"

Categories: Large Structures | Saccharomyces cerevisiae | Joshua-Tor L | Kumar PR

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:3btv.png|left|200px]]
-<!--
+==Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S0)-[G301R]==
-The line below this paragraph, containing "STRUCTURE_3btv", creates the "Structure Box" on the page.
+<StructureSection load='3btv' size='340' side='right'caption='[[3btv]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3btv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BTV FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3btv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3btv OCA], [https://pdbe.org/3btv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3btv RCSB], [https://www.ebi.ac.uk/pdbsum/3btv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3btv ProSAT]</span></td></tr>
-{{STRUCTURE_3btv|  PDB=3btv  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bt/3btv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3btv ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
-===Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S0)-[G301R]===
+NADP regulates the yeast GAL induction system.,Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L Science. 2008 Feb 22;319(5866):1090-2. PMID:18292341<ref>PMID:18292341</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3btv" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18292341}}, adds the Publication Abstract to the page
+*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18292341 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18292341}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-BTV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BTV OCA].
-==Reference==
-NADP regulates the yeast GAL induction system., Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L, Science. 2008 Feb 22;319(5866):1090-2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18292341 18292341]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Joshua-Tor L]]
-[[Category: Joshua-Tor, L.]]
+[[Category: Kumar PR]]
-[[Category: Kumar, P R.]]
-[[Category: Acetylation]]
-[[Category: Carbohydrate metabolism]]
-[[Category: Dna-binding]]
-[[Category: Eukaryotic transcription repressor]]
-[[Category: Galactose metabolism]]
-[[Category: Transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:50:52 2008''

3btv

From Proteopedia

Current revision

Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S0)-[G301R]

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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