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3c4q

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(New page: 200px {{Structure |PDB= 3c4q |SIZE=350|CAPTION= <scene name='initialview01'>3c4q</scene>, resolution 2.80&Aring; |SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+F...)
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[[Image:3c4q.jpg|left|200px]]
 
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{{Structure
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==Structure of the retaining glycosyltransferase MshA : The first step in mycothiol biosynthesis. Organism : Corynebacterium glutamicum- Complex with UDP==
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|PDB= 3c4q |SIZE=350|CAPTION= <scene name='initialview01'>3c4q</scene>, resolution 2.80&Aring;
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<StructureSection load='3c4q' size='340' side='right'caption='[[3c4q]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+602'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Residue+B+602'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+603'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+B+603'>AC4</scene>, <scene name='pdbsite=AC5:Udp+Binding+Site+For+Residue+A+600'>AC5</scene> and <scene name='pdbsite=AC6:Udp+Binding+Site+For+Residue+B+600'>AC6</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5&#39;-DIPHOSPHATE'>UDP</scene>
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<table><tr><td colspan='2'>[[3c4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C4Q FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE= MshA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 Corynebacterium glutamicum])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00534 Glycos_transf_1], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0438 RfaG]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4q OCA], [https://pdbe.org/3c4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c4q RCSB], [https://www.ebi.ac.uk/pdbsum/3c4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4q ProSAT]</span></td></tr>
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|RELATEDENTRY=[[3c48|3C48]], [[3c4v|3C4V]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4q OCA], [http://www.ebi.ac.uk/pdbsum/3c4q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3c4q RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MSHA_CORGL MSHA_CORGL] Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.<ref>PMID:18390549</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/3c4q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c4q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The glycosyltransferase termed MshA catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to 1-L-myo-inositol-1-phosphate in the first committed step of mycothiol biosynthesis. The structure of MshA from Corynebacterium glutamicum was determined both in the absence of substrates and in a complex with UDP and 1-L-myo-inositol-1-phosphate. MshA belongs to the GT-B structural family whose members have a two-domain structure with both domains exhibiting a Rossman-type fold. Binding of the donor sugar to the C-terminal domain produces a 97 degrees rotational reorientation of the N-terminal domain relative to the C-terminal domain, clamping down on UDP and generating the binding site for 1-L-myo-inositol-1-phosphate. The structure highlights the residues important in binding of UDP-N-acetylglucosamine and 1-L-myo-inositol-1-phosphate. Molecular models of the ternary complex suggest a mechanism in which the beta-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attack of the 3-hydroxyl group of 1-L-myo-inositol-1-phosphate while at the same time promoting the cleavage of the sugar nucleotide bond.
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'''Structure of the retaining glycosyltransferase MshA : The first step in mycothiol biosynthesis. Organism : Corynebacterium glutamicum- Complex with UDP'''
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Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis.,Vetting MW, Frantom PA, Blanchard JS J Biol Chem. 2008 Jun 6;283(23):15834-44. Epub 2008 Apr 4. PMID:18390549<ref>PMID:18390549</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3C4Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4Q OCA].
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<div class="pdbe-citations 3c4q" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Corynebacterium glutamicum]]
[[Category: Corynebacterium glutamicum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Blanchard, J S.]]
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[[Category: Blanchard JS]]
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[[Category: Frantom, P A.]]
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[[Category: Frantom PA]]
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[[Category: Vetting, M W.]]
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[[Category: Vetting MW]]
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[[Category: beta alpha beta]]
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[[Category: retaining glycosyltransferase]]
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[[Category: substrate assisted catalysis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:59:58 2008''
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Current revision

Structure of the retaining glycosyltransferase MshA : The first step in mycothiol biosynthesis. Organism : Corynebacterium glutamicum- Complex with UDP

PDB ID 3c4q

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