This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3cqa

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:32, 30 August 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3cqa.png|left|200px]]
 
-
<!--
+
==Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ala and Lys101Ala==
-
The line below this paragraph, containing "STRUCTURE_3cqa", creates the "Structure Box" on the page.
+
<StructureSection load='3cqa' size='340' side='right'caption='[[3cqa]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3cqa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CQA FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_3cqa| PDB=3cqa | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cqa OCA], [https://pdbe.org/3cqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cqa RCSB], [https://www.ebi.ac.uk/pdbsum/3cqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cqa ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/3cqa_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cqa ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Large-volume protein crystals are a prerequisite for neutron diffraction studies and their production represents a bottleneck in obtaining neutron structures. Many protein crystals that permit the collection of high-resolution X-ray diffraction data are inappropriate for neutron diffraction owing to a plate-type morphology that limits the crystal volume. Human fibroblast growth factor 1 crystallizes in a plate morphology that yields atomic resolution X-ray diffraction data but has insufficient volume for neutron diffraction. The thin physical dimension has been identified as corresponding to the b cell edge and the X-ray structure identified a solvent-mediated crystal contact adjacent to position Glu81 that was hypothesized to limit efficient crystal growth in this dimension. In this report, a series of mutations at this crystal contact designed to both reduce side-chain entropy and replace the solvent-mediated interface with direct side-chain contacts are reported. The results suggest that improved crystal growth is achieved upon the introduction of direct crystal contacts, while little improvement is observed with side-chain entropy-reducing mutations alone.
-
===Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ala and Lys101Ala===
+
Engineering an improved crystal contact across a solvent-mediated interface of human fibroblast growth factor 1.,Meher AK, Blaber SI, Lee J, Honjo E, Kuroki R, Blaber M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt, 11):1136-40. Epub 2009 Oct 30. PMID:19923735<ref>PMID:19923735</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3cqa" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19923735}}, adds the Publication Abstract to the page
+
*[[Fibroblast growth factor 3D structures|Fibroblast growth factor 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19923735 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19923735}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
3CQA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CQA OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:19923735</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Blaber, M.]]
+
[[Category: Large Structures]]
-
[[Category: Honjo, E.]]
+
[[Category: Blaber M]]
-
[[Category: Kuroki, R.]]
+
[[Category: Honjo E]]
-
[[Category: Lee, J.]]
+
[[Category: Kuroki R]]
-
[[Category: Meher, A K.]]
+
[[Category: Lee J]]
-
[[Category: Somasundaram, T.]]
+
[[Category: Meher AK]]
-
[[Category: Acetylation]]
+
[[Category: Somasundaram T]]
-
[[Category: Angiogenesis]]
+
-
[[Category: Crystal packing]]
+
-
[[Category: Developmental protein]]
+
-
[[Category: Differentiation]]
+
-
[[Category: Growth factor]]
+
-
[[Category: Heparin-binding]]
+
-
[[Category: Hormone]]
+
-
[[Category: Mitogen]]
+
-
[[Category: Polymorphism]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 6 09:00:18 2010''
+

Current revision

Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ala and Lys101Ala

PDB ID 3cqa

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cqa"
Personal tools