This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3cr8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:32, 30 August 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3cr8.png|left|200px]]
 
-
<!--
+
==Hexameric APS kinase from Thiobacillus denitrificans==
-
The line below this paragraph, containing "STRUCTURE_3cr8", creates the "Structure Box" on the page.
+
<StructureSection load='3cr8' size='340' side='right'caption='[[3cr8]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3cr8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thiobacillus_denitrificans Thiobacillus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CR8 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cr8 OCA], [https://pdbe.org/3cr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cr8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cr8 ProSAT]</span></td></tr>
-
{{STRUCTURE_3cr8| PDB=3cr8 | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q3SM86_THIDA Q3SM86_THIDA]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/3cr8_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cr8 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The Tbd_0210 gene of the chemolithotrophic bacterium Thiobacillus denitrificans is annotated to encode a 60.5 kDa bifunctional enzyme with ATP sulfurylase and APS kinase activity. This putative bifunctional enzyme was cloned, expressed and structurally characterized. The 2.95 A resolution X-ray crystal structure reported here revealed a hexameric assembly with D(3) symmetry. Each subunit contains a large N-terminal sulfurylase-like domain and a C-terminal APS kinase domain reminiscent of the two-domain fungal ATP sulfurylases of Penicillium chrysogenum and Saccharomyces cerevisiae, which also exhibit a hexameric assembly. However, the T. denitrificans enzyme exhibits numerous structural and sequence differences in the N-terminal domain that render it inactive with respect to ATP sulfurylase activity. Surprisingly, the C-terminal domain does indeed display APS kinase activity, indicating that this gene product is a true APS kinase. Therefore, these results provide the first structural insights into a unique hexameric APS kinase that contains a nonfunctional ATP sulfurylase-like domain of unknown function.
-
===Hexameric APS kinase from Thiobacillus denitrificans===
+
Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity.,Gay SC, Segel IH, Fisher AJ Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1021-31. Epub 2009, Sep 16. PMID:19770499<ref>PMID:19770499</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19770499}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3cr8" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 19770499 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19770499}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
3CR8 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Thiobacillus_denitrificans Thiobacillus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CR8 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:19770499</ref><references group="xtra"/>
+
[[Category: Thiobacillus denitrificans]]
[[Category: Thiobacillus denitrificans]]
-
[[Category: Fisher, A J.]]
+
[[Category: Fisher AJ]]
-
[[Category: Gay, S C.]]
+
[[Category: Gay SC]]
-
[[Category: Segel, I H.]]
+
[[Category: Segel IH]]
-
[[Category: Adenylylsulfate kinase]]
+
-
[[Category: Aps kinase]]
+
-
[[Category: Nucleotide 2 kinase]]
+
-
[[Category: Nucleotidyltransferase]]
+
-
[[Category: Sulfate metabolism]]
+
-
[[Category: Transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 30 14:17:14 2009''
+

Current revision

Hexameric APS kinase from Thiobacillus denitrificans

PDB ID 3cr8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cr8"
Personal tools