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3cwh

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{{Seed}}
 
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[[Image:3cwh.jpg|left|200px]]
 
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<!--
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==D-xylose Isomerase in complex with linear product, per-deuterated xylulose==
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The line below this paragraph, containing "STRUCTURE_3cwh", creates the "Structure Box" on the page.
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<StructureSection load='3cwh' size='340' side='right'caption='[[3cwh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CWH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=XUL:D-XYLULOSE'>XUL</scene></td></tr>
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{{STRUCTURE_3cwh| PDB=3cwh | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cwh OCA], [https://pdbe.org/3cwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cwh RCSB], [https://www.ebi.ac.uk/pdbsum/3cwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cwh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/3cwh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cwh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The time-of-flight neutron Laue technique has been used to determine the location of hydrogen atoms in the enzyme d-xylose isomerase (XI). The neutron structure of crystalline XI with bound product, d-xylulose, shows, unexpectedly, that O5 of d-xylulose is not protonated but is hydrogen-bonded to doubly protonated His54. Also, Lys289, which is neutral in native XI, is protonated (positively charged), while the catalytic water in native XI has become activated to a hydroxyl anion which is in the proximity of C1 and C2, the molecular site of isomerization of xylose. These findings impact our understanding of the reaction mechanism.
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===D-xylose Isomerase in complex with linear product, per-deuterated xylulose===
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Hydrogen location in stages of an enzyme-catalyzed reaction: time-of-flight neutron structure of D-xylose isomerase with bound D-xylulose.,Kovalevsky AY, Katz AK, Carrell HL, Hanson L, Mustyakimov M, Fisher SZ, Coates L, Schoenborn BP, Bunick GJ, Glusker JP, Langan P Biochemistry. 2008 Jul 22;47(29):7595-7. Epub 2008 Jun 26. PMID:18578508<ref>PMID:18578508</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cwh" style="background-color:#fffaf0;"></div>
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<!--
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18578508}}, adds the Publication Abstract to the page
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*[[D-xylose isomerase 3D structures|D-xylose isomerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18578508 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18578508}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3CWH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWH OCA].
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==Reference==
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Hydrogen location in stages of an enzyme-catalyzed reaction: time-of-flight neutron structure of D-xylose isomerase with bound D-xylulose., Kovalevsky AY, Katz AK, Carrell HL, Hanson L, Mustyakimov M, Fisher SZ, Coates L, Schoenborn BP, Bunick GJ, Glusker JP, Langan P, Biochemistry. 2008 Jul 22;47(29):7595-7. Epub 2008 Jun 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18578508 18578508]
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[[Category: Single protein]]
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[[Category: Streptomyces rubiginosus]]
[[Category: Streptomyces rubiginosus]]
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[[Category: Xylose isomerase]]
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[[Category: Glusker JP]]
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[[Category: Glusker, J P.]]
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[[Category: Kovalevsky AY]]
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[[Category: Kovalevsky, A Y.]]
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[[Category: Langan P]]
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[[Category: Langan, P.]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Cytoplasm]]
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[[Category: Enzyme-product complex]]
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[[Category: Isomerase]]
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[[Category: Magnesium]]
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[[Category: Metal-binding]]
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[[Category: Pentose shunt]]
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[[Category: Xylose metabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 6 13:00:35 2008''
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Current revision

D-xylose Isomerase in complex with linear product, per-deuterated xylulose

PDB ID 3cwh

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