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8odw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of LbmA Ox-ACP didomain in complex with NADP and ethyl glycinate from the lobatamide PKS (Gynuella sunshinyii)== | |
| + | <StructureSection load='8odw' size='340' side='right'caption='[[8odw]], [[Resolution|resolution]] 3.07Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8odw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gynuella_sunshinyii Gynuella sunshinyii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ODW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ODW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.07Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GEE:ETHYL+GLYCINATE'>GEE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8odw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8odw OCA], [https://pdbe.org/8odw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8odw RCSB], [https://www.ebi.ac.uk/pdbsum/8odw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8odw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0C5VQJ2_9GAMM A0A0C5VQJ2_9GAMM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Modular trans-acyltransferase polyketide synthases (trans-AT PKSs) are enzymatic assembly lines that biosynthesize complex polyketide natural products. Relative to their better studied cis-AT counterparts, the trans-AT PKSs introduce remarkable chemical diversity into their polyketide products. A notable example is the lobatamide A PKS, which incorporates a methylated oxime. Here we demonstrate biochemically that this functionality is installed on-line by an unusual oxygenase-containing bimodule. Furthermore, analysis of the oxygenase crystal structure coupled with site-directed mutagenesis allows us to propose a model for catalysis, as well as identifying key protein-protein interactions that support this chemistry. Overall, our work adds oxime-forming machinery to the biomolecular toolbox available for trans-AT PKS engineering, opening the way to introducing such masked aldehyde functionalities into diverse polyketides. | ||
| - | + | Modular oxime formation by a trans-AT polyketide synthase.,Minas HA, Francois RMM, Hemmerling F, Fraley AE, Dieterich CL, Rudisser SH, Meoded RA, Collin S, Weissman KJ, Gruez A, Piel J Angew Chem Int Ed Engl. 2023 May 22:e202304481. doi: 10.1002/anie.202304481. PMID:37216334<ref>PMID:37216334</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8odw" style="background-color:#fffaf0;"></div> |
| - | [[Category: Francois | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Gynuella sunshinyii]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fraley AE]] | ||
| + | [[Category: Francois RMM]] | ||
| + | [[Category: Gruez A]] | ||
| + | [[Category: Piel J]] | ||
| + | [[Category: Weissman KJ]] | ||
Current revision
Crystal structure of LbmA Ox-ACP didomain in complex with NADP and ethyl glycinate from the lobatamide PKS (Gynuella sunshinyii)
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