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2bj6

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[[Image:2bj6.gif|left|200px]]<br />
 
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<applet load="2bj6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bj6, resolution 2.60&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A DECAMERIC HNA-RNA HYBRID'''<br />
 
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==Overview==
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==Crystal Structure of a decameric HNA-RNA hybrid==
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The crystal structure of a decameric HNA/RNA (HNA =, 2',3'-dideoxy-1',5'-anhydro-d-arabinohexitol nucleic acid) hybrid with the, RNA sequence 5'-GGCAUUACGG-3' is the first crystal structure of a hybrid, duplex between a naturally occurring nucleic acid and a strand, which is, fully modified to contain a six-membered ring instead of ribose. The, presence of four duplex helices in the asymmetric unit allows for a, detailed discussion of hydration, which revealed a tighter spinelike, backbone hydration for the HNA- than for the RNA-strands. The reinforced, backbone hydration is suggested to contribute significantly to the, exceptional stability of HNA-containing duplexes and might be one of the, causes for the evolutionary preference for ribose-derived nucleic acids.
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<StructureSection load='2bj6' size='340' side='right'caption='[[2bj6]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bj6]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJ6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6HA:1,5-ANHYDRO-2,3-DIDEOXY-2-(ADENIN-9-YL)-6-O-PHOSPHORYL-D-ARABINO-HEXITOL'>6HA</scene>, <scene name='pdbligand=6HC:1,5-ANHYDRO-2,3-DIDEOXY-2-(CYTOSIN-1-YL)-6-O-PHOSPHORYL-D-ARABINO-HEXITOL'>6HC</scene>, <scene name='pdbligand=6HG:1,5-ANHYDRO-2,3-DIDEOXY-2-(GUANIN-9-YL)-6-O-PHOSPHORYL-D-ARABINO-HEXITOL'>6HG</scene>, <scene name='pdbligand=6HT:1,5-ANHYDRO-2,3-DIDEOXY-2-(THYMIN-1-YL)-6-O-PHOSPHORYL-D-ARABINO-HEXITOL'>6HT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bj6 OCA], [https://pdbe.org/2bj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bj6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bj6 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a decameric HNA/RNA (HNA = 2',3'-dideoxy-1',5'-anhydro-d-arabinohexitol nucleic acid) hybrid with the RNA sequence 5'-GGCAUUACGG-3' is the first crystal structure of a hybrid duplex between a naturally occurring nucleic acid and a strand, which is fully modified to contain a six-membered ring instead of ribose. The presence of four duplex helices in the asymmetric unit allows for a detailed discussion of hydration, which revealed a tighter spinelike backbone hydration for the HNA- than for the RNA-strands. The reinforced backbone hydration is suggested to contribute significantly to the exceptional stability of HNA-containing duplexes and might be one of the causes for the evolutionary preference for ribose-derived nucleic acids.
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==About this Structure==
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Reinforced HNA backbone hydration in the crystal structure of a decameric HNA/RNA hybrid.,Maier T, Przylas I, Strater N, Herdewijn P, Saenger W J Am Chem Soc. 2005 Mar 9;127(9):2937-43. PMID:15740130<ref>PMID:15740130</ref>
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2BJ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJ6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Reinforced HNA backbone hydration in the crystal structure of a decameric HNA/RNA hybrid., Maier T, Przylas I, Strater N, Herdewijn P, Saenger W, J Am Chem Soc. 2005 Mar 9;127(9):2937-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15740130 15740130]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2bj6" style="background-color:#fffaf0;"></div>
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[[Category: Herdewijn, P.]]
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== References ==
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[[Category: Maier, T.]]
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<references/>
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[[Category: Przylas, I.]]
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__TOC__
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[[Category: Saenger, W.]]
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</StructureSection>
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[[Category: Straeter, N.]]
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[[Category: Homo sapiens]]
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[[Category: SO4]]
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[[Category: Large Structures]]
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[[Category: antisense]]
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[[Category: Herdewijn P]]
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[[Category: backbone modification]]
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[[Category: Maier T]]
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[[Category: dna]]
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[[Category: Przylas I]]
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[[Category: duplex]]
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[[Category: Saenger W]]
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[[Category: hexitol nucleic acid]]
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[[Category: Straeter N]]
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[[Category: hna]]
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[[Category: hybrid]]
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[[Category: modified nucleic acid]]
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[[Category: rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:32:02 2007''
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Current revision

Crystal Structure of a decameric HNA-RNA hybrid

PDB ID 2bj6

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