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5imk

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Nanobody targeting human Vsig4 in Spacegroup C2

Structural highlights

5imk is a 2 chain structure with sequence from Camelidae and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.227Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VSIG4_HUMAN Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.^[1] ^[2]

Publication Abstract from PubMed

Vsig4 is a recently identified immune regulatory protein related to the B7 family with dual functionality: a negative regulator of T cell activation and a receptor for the complement components C3b and C3c. Here we present a structural evaluation of a nanobody, Nb119, against the extracellular IgV domain protein of both mouse and human recombinant Vsig4, which have a high degree of sequence identity. Although mouse and human Vsig4 bind to Nb119 with a 250 times difference in dissociation constants, the interaction results in a highly identical assembly with a RMSD of 0.4A. The molecular determinants for Vsig4 recognition and cross reactivity unveiled by the atomic structure of Nb119 in complex with mVsig4 and hVsig4 afford new insights useful for the further optimization of the nanobody for potential use in humans. Additionally, structural analysis of the Vsig4-Nb119 complexes indicates that Nb119 occupies the interface on Vsig4 recognized by the macroglobulin-like domains MG4 and MG5 of C3b. Thus an affinity-improved Nb119 may have the potential to influence the activation of both T cells and complement.

Structural evaluation of a nanobody targeting complement receptor Vsig4 and its cross reactivity.,Wen Y, Ouyang Z, Schoonooghe S, Luo S, De Baetselier P, Lu W, Muyldermans S, Raes G, Zheng F Immunobiology. 2016 Nov 18. pii: S0171-2985(16)30436-3. doi:, 10.1016/j.imbio.2016.11.008. PMID:27889311^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vogt L, Schmitz N, Kurrer MO, Bauer M, Hinton HI, Behnke S, Gatto D, Sebbel P, Beerli RR, Sonderegger I, Kopf M, Saudan P, Bachmann MF. VSIG4, a B7 family-related protein, is a negative regulator of T cell activation. J Clin Invest. 2006 Oct;116(10):2817-26. PMID:17016562 doi:10.1172/JCI25673
↑ Wiesmann C, Katschke KJ, Yin J, Helmy KY, Steffek M, Fairbrother WJ, McCallum SA, Embuscado L, DeForge L, Hass PE, van Lookeren Campagne M. Structure of C3b in complex with CRIg gives insights into regulation of complement activation. Nature. 2006 Nov 9;444(7116):217-20. Epub 2006 Oct 15. PMID:17051150 doi:10.1038/nature05263
↑ Wen Y, Ouyang Z, Schoonooghe S, Luo S, De Baetselier P, Lu W, Muyldermans S, Raes G, Zheng F. Structural evaluation of a nanobody targeting complement receptor Vsig4 and its cross reactivity. Immunobiology. 2016 Nov 18. pii: S0171-2985(16)30436-3. doi:, 10.1016/j.imbio.2016.11.008. PMID:27889311 doi:http://dx.doi.org/10.1016/j.imbio.2016.11.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5imk"

Categories: Camelidae | Homo sapiens | Large Structures | Wen Y

@@ Line 1: / Line 1: @@
 ==Nanobody targeting human Vsig4 in Spacegroup C2==
-<StructureSection load='5imk' size='340' side='right' caption='[[5imk]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
+<StructureSection load='5imk' size='340' side='right'caption='[[5imk]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5imk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelidae Camelidae] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IMK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5imk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelidae Camelidae] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IMK FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iml|5iml]], [[5imm|5imm]], [[5imo|5imo]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.227&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VSIG4, CRIg, Z39IG, UNQ317/PRO362 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5imk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5imk OCA], [https://pdbe.org/5imk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5imk RCSB], [https://www.ebi.ac.uk/pdbsum/5imk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5imk ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5imk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5imk OCA], [http://pdbe.org/5imk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5imk RCSB], [http://www.ebi.ac.uk/pdbsum/5imk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5imk ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VSIG4_HUMAN VSIG4_HUMAN]] Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.<ref>PMID:17016562</ref> <ref>PMID:17051150</ref>
+[https://www.uniprot.org/uniprot/VSIG4_HUMAN VSIG4_HUMAN] Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.<ref>PMID:17016562</ref> <ref>PMID:17051150</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 23: @@
 </StructureSection>
 [[Category: Camelidae]]
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Wen, Y]]
+[[Category: Large Structures]]
-[[Category: Complement receptor]]
+[[Category: Wen Y]]
-[[Category: Immune system]]
-[[Category: Nanobody]]
-[[Category: Vsig4 crig]]

5imk

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Current revision

Nanobody targeting human Vsig4 in Spacegroup C2

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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