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5imq
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of ribosome bound to cofactor at 3.8 angstrom resolution== | |
| + | <SX load='5imq' size='340' side='right' viewer='molstar' caption='[[5imq]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5imq]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Enteritidis_str._SA20094389 Salmonella enterica subsp. enterica serovar Enteritidis str. SA20094389] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IMQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IMQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5imq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5imq OCA], [https://pdbe.org/5imq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5imq RCSB], [https://www.ebi.ac.uk/pdbsum/5imq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5imq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RL36_THET8 RL36_THET8] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Elongation factor 4 (EF4) is a member of the family of ribosome-dependent translational GTPase (trGTPase) factors, along with elongation factor G (EF-G) and BPI-inducible protein A (BipA). Although EF4 is highly conserved in bacterial, mitochondrial, and chloroplast genomes, its exact biological function remains controversial. Here, we present the cryo-EM reconstitution of the GTP form of EF4 bound to the ribosome with P- and E-site tRNAs at 3.8 A resolution. Interestingly, our structure reveals an unrotated ribosome rather than a clockwise-rotated ribosome, as observed in the presence of EF4-GDP and P-site tRNA. In addition, we also observed an counterclockwise rotated form of the above complex at 5.7 A resolution. Taken together, our results shed light on the interactions formed between EF4, the ribosome, and the P-site tRNA and illuminate the GTPase activation mechanism at previously unresolved detail. | ||
| - | + | Structure of the GTP form of elongation factor 4 (EF4) bound to the ribosome.,Kumar V, Ero R, Ahmed T, Goh KJ, Zhan Y, Bhushan S, Gao YG J Biol Chem. 2016 May 2. pii: jbc.M116.725945. PMID:27137929<ref>PMID:27137929</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5imq" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Elongation factor 3D structures|Elongation factor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Salmonella enterica subsp. enterica serovar Enteritidis str. SA20094389]] | ||
| + | [[Category: Thermus thermophilus HB8]] | ||
| + | [[Category: Ahmed T]] | ||
| + | [[Category: Bhushan S]] | ||
| + | [[Category: Ero R]] | ||
| + | [[Category: Gao YG]] | ||
| + | [[Category: Jian GK]] | ||
| + | [[Category: Kumar V]] | ||
| + | [[Category: Zhan Y]] | ||
Current revision
Structure of ribosome bound to cofactor at 3.8 angstrom resolution
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