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3fd4

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Crystal Structure of Epstein-Barr virus gp42 protein

Structural highlights

3fd4 is a 2 chain structure with sequence from Human herpesvirus 4 strain B95-8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GP42_EBVB9 Plays a role in virion attachment to host B-lymphocytes, through binding to leukocyte antigen (HLA) class II and subsequently participates in fusion of the virion with host membranes. May act as a tropism switch that directs fusion with B-lymphocytes and inhibits fusion with epithelial cells.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Epstein-Barr virus requires glycoproteins gH/gL, gB, and gp42 to fuse its lipid envelope with B cells. Gp42 is a type II membrane protein consisting of a flexible N-terminal region, which binds gH/gL, and a C-terminal lectin-like domain that binds to the B-cell entry receptor human leukocyte antigen (HLA) class II. Gp42 triggers membrane fusion after HLA binding, a process that requires simultaneous binding to gH/gL and a functional hydrophobic pocket in the lectin domain adjacent to the HLA binding site. Here we present the structure of gp42 in its unbound form. Comparisons to the previously determined structure of a gp42:HLA complex reveals additional N-terminal residues forming part of the gH/gL binding site and structural changes in the receptor binding domain. Although the core of the lectin domain remains similar, significant shifts in two loops and an alpha helix bordering the essential hydrophobic pocket suggest a structural mechanism for triggering fusion.

Structure of Epstein-Barr virus glycoprotein 42 suggests a mechanism for triggering receptor-activated virus entry.,Kirschner AN, Sorem J, Longnecker R, Jardetzky TS Structure. 2009 Feb 13;17(2):223-33. PMID:19217393^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Borza CM, Hutt-Fletcher LM. Alternate replication in B cells and epithelial cells switches tropism of Epstein-Barr virus. Nat Med. 2002 Jun;8(6):594-9. PMID:12042810 doi:10.1038/nm0602-594
↑ Kirschner AN, Sorem J, Longnecker R, Jardetzky TS. Structure of Epstein-Barr virus glycoprotein 42 suggests a mechanism for triggering receptor-activated virus entry. Structure. 2009 Feb 13;17(2):223-33. PMID:19217393 doi:10.1016/j.str.2008.12.010

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fd4"

Categories: Human herpesvirus 4 strain B95-8 | Large Structures | Jardetzky T | Kirschner A

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Epstein-Barr virus gp42 protein==
-<StructureSection load='3fd4' size='340' side='right' caption='[[3fd4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='3fd4' size='340' side='right'caption='[[3fd4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3fd4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebvb9 Ebvb9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FD4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3fd4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FD4 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BZLF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10377 EBVB9])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fd4 OCA], [http://pdbe.org/3fd4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fd4 RCSB], [http://www.ebi.ac.uk/pdbsum/3fd4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fd4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fd4 OCA], [https://pdbe.org/3fd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fd4 RCSB], [https://www.ebi.ac.uk/pdbsum/3fd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fd4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GP42_EBVB9 GP42_EBVB9]] Plays a role in virion attachment to host B-lymphocytes, through binding to leukocyte antigen (HLA) class II and subsequently participates in fusion of the virion with host membranes. May act as a tropism switch that directs fusion with B-lymphocytes and inhibits fusion with epithelial cells.<ref>PMID:12042810</ref>
+[https://www.uniprot.org/uniprot/GP42_EBVB9 GP42_EBVB9] Plays a role in virion attachment to host B-lymphocytes, through binding to leukocyte antigen (HLA) class II and subsequently participates in fusion of the virion with host membranes. May act as a tropism switch that directs fusion with B-lymphocytes and inhibits fusion with epithelial cells.<ref>PMID:12042810</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fd4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fd4_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Ebvb9]]
+[[Category: Human herpesvirus 4 strain B95-8]]
-[[Category: Jardetzky, T]]
+[[Category: Large Structures]]
-[[Category: Kirschner, A]]
+[[Category: Jardetzky T]]
-[[Category: C type lectin]]
+[[Category: Kirschner A]]
-[[Category: Herpesvirus]]
-[[Category: Host-virus interaction]]
-[[Category: Lectin]]
-[[Category: Membrane]]
-[[Category: Membrane fusion]]
-[[Category: Transmembrane]]
-[[Category: Viral protein]]
-[[Category: Virus entry]]

3fd4

From Proteopedia

Current revision

Crystal Structure of Epstein-Barr virus gp42 protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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