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| | ==Crystal structure of ACBP from Moniliophthora perniciosa== | | ==Crystal structure of ACBP from Moniliophthora perniciosa== |
| - | <StructureSection load='3fp5' size='340' side='right' caption='[[3fp5]], [[Resolution|resolution]] 1.61Å' scene=''> | + | <StructureSection load='3fp5' size='340' side='right'caption='[[3fp5]], [[Resolution|resolution]] 1.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3fp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crinipellis_perniciosa Crinipellis perniciosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FP5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3fp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moniliophthora_perniciosa Moniliophthora perniciosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FP5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MpACBP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=153609 Crinipellis perniciosa])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fp5 OCA], [http://pdbe.org/3fp5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3fp5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fp5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fp5 OCA], [https://pdbe.org/3fp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3fp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fp5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D1MPT1_MONPR D1MPT1_MONPR] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Crinipellis perniciosa]] | + | [[Category: Large Structures]] |
| - | [[Category: Cascardo, J C.M]]
| + | |
| - | [[Category: Meirelles, F V]]
| + | |
| - | [[Category: Melo, F A]]
| + | |
| - | [[Category: Monzani, P S]]
| + | |
| - | [[Category: Oliva, G]]
| + | |
| - | [[Category: Pereira, H M]]
| + | |
| - | [[Category: Acbp]]
| + | |
| - | [[Category: Cacao disease]]
| + | |
| - | [[Category: Fatty acid metabolism]]
| + | |
| - | [[Category: Lipid binding protein]]
| + | |
| | [[Category: Moniliophthora perniciosa]] | | [[Category: Moniliophthora perniciosa]] |
| | + | [[Category: Cascardo JCM]] |
| | + | [[Category: Meirelles FV]] |
| | + | [[Category: Melo FA]] |
| | + | [[Category: Monzani PS]] |
| | + | [[Category: Oliva G]] |
| | + | [[Category: Pereira HM]] |
| Structural highlights
Function
D1MPT1_MONPR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Acyl-CoA binding protein (ACBP) is a housekeeping protein and is an essential protein in human cell lines and in Trypanosoma brucei. The ACBP of Moniliophthora perniciosa is composed of 104 amino acids and is possibly a non-classic isoform exclusively from Basidiomycetes. The M. perniciosa acbp gene was cloned, and the protein was expressed and purified. Acyl-CoA ester binding was analyzed by isoelectric focusing, native gel electrophoresis and isothermal titration calorimetry. Our results suggest an increasing affinity of ACBP for longer acyl-CoA esters, such as myristoyl-CoA to arachidoyl-CoA, and best fit modeling indicates two binding sites. ACBP undergoes a shift from a monomeric to a dimeric state, as shown by dynamic light scattering, fluorescence anisotropy and native gel electrophoresis in the absence and presence of the ligand. The protein's structure was determined at 1.6 A resolution and revealed a new topology for ACBP, containing five alpha-helices instead of four. alpha-helices 1, 2, 3 and 4 adopted a bundled arrangement that is unique from the previously determined four-helix folds of ACBP, while alpha-helices 1, 2, 4 and 5 formed a classical four-helix bundle. A MES molecule was found in the CoA binding site, suggesting that the CoA site could be a target for small compound screening.
A new topology of ACBP from Moniliophthora perniciosa.,Monzani PS, Pereira HM, Melo FA, Meirelles FV, Oliva G, Cascardo JC Biochim Biophys Acta. 2010 Jan;1804(1):115-23. Epub 2009 Sep 24. PMID:19782157[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Monzani PS, Pereira HM, Melo FA, Meirelles FV, Oliva G, Cascardo JC. A new topology of ACBP from Moniliophthora perniciosa. Biochim Biophys Acta. 2010 Jan;1804(1):115-23. Epub 2009 Sep 24. PMID:19782157 doi:10.1016/j.bbapap.2009.09.020
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