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3hqc
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==Crystal structure of Phosphotyrosine-binding domain from the Human Tensin-like C1 domain-containing phosphatase (TENC1)== | ==Crystal structure of Phosphotyrosine-binding domain from the Human Tensin-like C1 domain-containing phosphatase (TENC1)== | ||
| - | <StructureSection load='3hqc' size='340' side='right' caption='[[3hqc]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3hqc' size='340' side='right'caption='[[3hqc]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3hqc]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3hqc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HQC FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | < | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hqc OCA], [https://pdbe.org/3hqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hqc RCSB], [https://www.ebi.ac.uk/pdbsum/3hqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hqc ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/3hqc TOPSAN]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/TENS2_HUMAN TENS2_HUMAN] Tyrosine-protein phosphatase which regulates cell motility, proliferation and muscle-response to insulin (PubMed:15817639, PubMed:23401856). Phosphatase activity is mediated by binding to phosphatidylinositol-3,4,5-triphosphate (PtdIns(3,4,5)P3) via the SH2 domain (PubMed:30092354). In muscles and under catabolic conditions, dephosphorylates IRS1 leading to its degradation and muscle atrophy (PubMed:23401856, PubMed:30092354). Negatively regulates PI3K-AKT pathway activation (PubMed:15817639, PubMed:23401856, PubMed:30092354). Dephosphorylates nephrin NPHS1 in podocytes which regulates activity of the mTORC1 complex (PubMed:28955049). Under normal glucose conditions, NPHS1 outcompetes IRS1 for binding to phosphatidylinositol 3-kinase (PI3K) which balances mTORC1 activity but high glucose conditions lead to up-regulation of TNS2, increased NPHS1 dephosphorylation and activation of mTORC1, contributing to podocyte hypertrophy and proteinuria (PubMed:28955049). Required for correct podocyte morphology, podocyte-glomerular basement membrane interaction and integrity of the glomerular filtration barrier (By similarity). Enhances RHOA activation in the presence of DLC1 (PubMed:26427649). Plays a role in promoting DLC1-dependent remodeling of the extracellular matrix (PubMed:20069572).[UniProtKB:Q8CGB6]<ref>PMID:15817639</ref> <ref>PMID:20069572</ref> <ref>PMID:23401856</ref> <ref>PMID:26427649</ref> <ref>PMID:28955049</ref> <ref>PMID:30092354</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Homo sapiens]] |
| - | [[Category: Atwell | + | [[Category: Large Structures]] |
| - | [[Category: Bain | + | [[Category: Atwell S]] |
| - | [[Category: Burley | + | [[Category: Bain K]] |
| - | [[Category: Dickey | + | [[Category: Burley SK]] |
| - | [[Category: Do | + | [[Category: Dickey M]] |
| - | [[Category: Gheyi | + | [[Category: Do J]] |
| - | [[Category: Klemke | + | [[Category: Gheyi T]] |
| - | + | [[Category: Klemke R]] | |
| - | [[Category: Romero | + | [[Category: Romero R]] |
| - | [[Category: Sampathkumar | + | [[Category: Sampathkumar P]] |
| - | [[Category: Sauder | + | [[Category: Sauder JM]] |
| - | [[Category: Wasserman | + | [[Category: Wasserman S]] |
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Current revision
Crystal structure of Phosphotyrosine-binding domain from the Human Tensin-like C1 domain-containing phosphatase (TENC1)
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Categories: Homo sapiens | Large Structures | Atwell S | Bain K | Burley SK | Dickey M | Do J | Gheyi T | Klemke R | Romero R | Sampathkumar P | Sauder JM | Wasserman S
