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3hts

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{{Seed}}
 
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[[Image:3hts.png|left|200px]]
 
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<!--
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==HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX==
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The line below this paragraph, containing "STRUCTURE_3hts", creates the "Structure Box" on the page.
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<StructureSection load='3hts' size='340' side='right'caption='[[3hts]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hts]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HTS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3hts| PDB=3hts | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hts OCA], [https://pdbe.org/3hts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hts RCSB], [https://www.ebi.ac.uk/pdbsum/3hts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hts ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HSF_KLULA HSF_KLULA] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/3hts_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hts ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 1.75 A crystal structure of the Kluyveromyces lactis heat shock transcription factor (HSF) DNA-binding domain (DBD) complexed with DNA reveals a protein-DNA interface with few direct major groove contacts and a number of phosphate backbone contacts that are primarily water-mediated interactions. The DBD, a 'winged' helix-turn-helix protein, displays a novel mode of binding in that the 'wing' does not contact DNA like all others of that class. Instead, the monomeric DBD, which crystallized as a symmetric dimer to a pair of nGAAn inverted repeats, uses the 'wing' to form part of the protein-protein contacts. This dimer interface is likely important for increasing the DNA-binding specificity and affinity of the trimeric form of HSF, as well as for increasing cooperativity between adjacent trimers.
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===HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX===
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A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal.,Littlefield O, Nelson HC Nat Struct Biol. 1999 May;6(5):464-70. PMID:10331875<ref>PMID:10331875</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hts" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10331875}}, adds the Publication Abstract to the page
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*[[Heat shock factor|Heat shock factor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10331875 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10331875}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3HTS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HTS OCA].
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==Reference==
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<ref group="xtra">PMID:10331875</ref><references group="xtra"/>
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[[Category: Kluyveromyces lactis]]
[[Category: Kluyveromyces lactis]]
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[[Category: Littlefield, O.]]
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[[Category: Large Structures]]
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[[Category: Nelson, H C.M.]]
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[[Category: Littlefield O]]
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[[Category: Dna-binding protein]]
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[[Category: Nelson HCM]]
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[[Category: Transcription regulation]]
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[[Category: Transcription/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 11:00:43 2009''
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Current revision

HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX

PDB ID 3hts

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