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3il2

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[[Image:3il2.png|left|200px]]
 
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==Crystal structure of a Rex-family repressor R90D mutant/DNA complex from Thermus aquaticus==
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The line below this paragraph, containing "STRUCTURE_3il2", creates the "Structure Box" on the page.
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<StructureSection load='3il2' size='340' side='right'caption='[[3il2]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3il2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IL2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3il2| PDB=3il2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3il2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3il2 OCA], [https://pdbe.org/3il2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3il2 RCSB], [https://www.ebi.ac.uk/pdbsum/3il2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3il2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/REX_THET2 REX_THET2] Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/il/3il2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3il2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nicotinamide adenine dinucleotides have emerged as key signals of the cellular redox state. Yet the structural basis for allosteric gene regulation by the ratio of reduced NADH to oxidized NAD(+) is poorly understood. A key sensor among Gram-positive bacteria, Rex represses alternative respiratory gene expression until a limited oxygen supply elevates the intracellular NADH:NAD(+) ratio. Here we investigate the molecular mechanism for NADH/NAD(+) sensing among Rex family members by determining structures of Thermus aquaticus Rex bound to (1) NAD(+), (2) DNA operator, and (3) without ligand. Comparison with the Rex/NADH complex reveals that NADH releases Rex from the DNA site following a 40 degrees closure between the dimeric subunits. Complementary site-directed mutagenesis experiments implicate highly conserved residues in NAD-responsive DNA-binding activity. These rare views of a redox sensor in action establish a means for slight differences in the nicotinamide charge, pucker, and orientation to signal the redox state of the cell.
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===Crystal structure of a Rex-family repressor R90D mutant/DNA complex from Thermus aquaticus===
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Structural basis for NADH/NAD+ redox sensing by a Rex family repressor.,McLaughlin KJ, Strain-Damerell CM, Xie K, Brekasis D, Soares AS, Paget MS, Kielkopf CL Mol Cell. 2010 May 28;38(4):563-75. PMID:20513431<ref>PMID:20513431</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20513431}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3il2" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20513431 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20513431}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3il2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IL2 OCA].
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[[Category: Thermus thermophilus HB27]]
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[[Category: Kielkopf CL]]
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==Reference==
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[[Category: McLaughlin KJ]]
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<ref group="xtra">PMID:020513431</ref><references group="xtra"/>
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[[Category: Thermus thermophilus hb27]]
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[[Category: Kielkopf, C L.]]
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[[Category: McLaughlin, K J.]]
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[[Category: Dna binding protein]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Dna-binding]]
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[[Category: Mutant]]
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[[Category: Nad]]
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[[Category: Nicotinamide adenine dinucleotide]]
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[[Category: Redox-sensing]]
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[[Category: Repressor]]
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[[Category: Rex]]
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[[Category: Rossmann fold]]
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[[Category: Thermus aquaticus]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Winged helix]]
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Current revision

Crystal structure of a Rex-family repressor R90D mutant/DNA complex from Thermus aquaticus

PDB ID 3il2

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