3inz

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{{Seed}}
 
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[[Image:3inz.jpg|left|200px]]
 
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==H57T Hfq from Pseudomonas aeruginosa==
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The line below this paragraph, containing "STRUCTURE_3inz", creates the "Structure Box" on the page.
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<StructureSection load='3inz' size='340' side='right'caption='[[3inz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3inz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3INZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3INZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3inz| PDB=3inz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3inz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3inz OCA], [https://pdbe.org/3inz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3inz RCSB], [https://www.ebi.ac.uk/pdbsum/3inz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3inz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/3inz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3inz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The bacterial Sm-like protein Hfq forms homohexamers both in solution and in crystals. The monomers are organized as a continuous beta-sheet passing through the whole hexamer ring with a common hydrophobic core. Analysis of the Pseudomonas aeruginosa Hfq (PaeHfq) hexamer structure suggested that solvent-inaccessible intermonomer hydrogen bonds created by conserved amino-acid residues should also stabilize the quaternary structure of the protein. In this work, one such conserved residue, His57, in PaeHfq was replaced by alanine, threonine or asparagine. The crystal structures of His57Thr and His57Ala Hfq were determined and the stabilities of all of the mutant forms and of the wild-type protein were measured. The results obtained demonstrate the great importance of solvent-inaccessible conserved hydrogen bonds between the Hfq monomers in stabilization of the hexamer structure.
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===H57T Hfq from Pseudomonas aeruginosa===
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The structures of mutant forms of Hfq from Pseudomonas aeruginosa reveal the importance of the conserved His57 for the protein hexamer organization.,Moskaleva O, Melnik B, Gabdulkhakov A, Garber M, Nikonov S, Stolboushkina E, Nikulin A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jul 1;66(Pt, 7):760-4. Epub 2010 Jun 23. PMID:20606268<ref>PMID:20606268</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3inz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20606268}}, adds the Publication Abstract to the page
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*[[Protein Hfq|Protein Hfq]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20606268 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20606268}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3INZ is a 6 chains structure with sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3INZ OCA].
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==Reference==
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<ref group="xtra">PMID:20606268</ref><ref group="xtra">PMID:15681864</ref><references group="xtra"/>
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Gabdulkhakov, A.]]
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[[Category: Gabdulkhakov A]]
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[[Category: Garber, M.]]
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[[Category: Garber M]]
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[[Category: Melnik, B.]]
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[[Category: Melnik B]]
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[[Category: Moskaleva, O.]]
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[[Category: Moskaleva O]]
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[[Category: Nikonov, S.]]
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[[Category: Nikonov S]]
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[[Category: Nikulin, A.]]
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[[Category: Nikulin A]]
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[[Category: Stolboushkina, E.]]
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[[Category: Stolboushkina E]]
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[[Category: Hfq]]
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[[Category: Protein tertiary structure]]
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[[Category: Rna-binding]]
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[[Category: Rna-binding protein]]
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[[Category: Stress response]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 4 09:06:41 2010''
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Current revision

H57T Hfq from Pseudomonas aeruginosa

PDB ID 3inz

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