3kzk

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{{Seed}}
 
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[[Image:3kzk.jpg|left|200px]]
 
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==Crystal structure of acetylornithine transcarbamylase complexed with acetylcitrulline==
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The line below this paragraph, containing "STRUCTURE_3kzk", creates the "Structure Box" on the page.
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<StructureSection load='3kzk' size='340' side='right'caption='[[3kzk]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris_str._ATCC_33913 Xanthomonas campestris pv. campestris str. ATCC 33913]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1yh1 1yh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KZK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=OLN:(S)-2-ACETAMIDO-5-UREIDOPENTANOIC+ACID'>OLN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3kzk| PDB=3kzk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kzk OCA], [https://pdbe.org/3kzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kzk RCSB], [https://www.ebi.ac.uk/pdbsum/3kzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kzk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AOTC_XANCP AOTC_XANCP] Catalyzes the conversion of N-acetylornithine to N-acetylcitrulline in an alternative arginine biosynthesis pathway. The enzyme has no activity with ornithine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/3kzk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kzk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have identified in Xanthomonas campestris a novel N-acetylornithine transcarbamylase that replaces ornithine transcarbamylase in the canonic arginine biosynthetic pathway of several Eubacteria. The crystal structures of the protein in the presence and absence of the reaction product, N-acetylcitrulline, were determined. This new family of transcarbamylases lacks the DxxSMG motif that is characteristic of all ornithine transcarbamylases (OTCases) and contains a novel proline-rich loop that forms part of the active site. The specificity for N-acetylornithine is conferred by hydrogen bonding with residues in the proline-rich loop via water molecules and by hydrophobic interactions with residues from the adjacent 80's, 120's, and proline-rich loops. This novel protein structure provides a starting point for rational design of specific analogs that may be useful in combating human and plant pathogens that utilize acetylornithine transcarbamylase rather than ornithine transcarbamylase.
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===Crystal structure of acetylornithine transcarbamylase complexed with acetylcitrulline===
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Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria.,Shi D, Morizono H, Yu X, Roth L, Caldovic L, Allewell NM, Malamy MH, Tuchman M J Biol Chem. 2005 Apr 15;280(15):14366-9. Epub 2005 Feb 24. PMID:15731101<ref>PMID:15731101</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3kzk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15731101}}, adds the Publication Abstract to the page
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*[[N-acetylornithine carbamoyltransferase|N-acetylornithine carbamoyltransferase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15731101 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15731101}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KZK is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1yh1 1yh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZK OCA].
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[[Category: Xanthomonas campestris pv. campestris str. ATCC 33913]]
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[[Category: Allewell NM]]
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==Reference==
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[[Category: Shi D]]
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<ref group="xtra">PMID:15731101</ref><references group="xtra"/>
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[[Category: Tuchman M]]
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[[Category: N-acetylornithine carbamoyltransferase]]
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[[Category: Yu X]]
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[[Category: Xanthomonas campestris pv. campestris]]
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[[Category: Allewell, N M.]]
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[[Category: Shi, D.]]
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[[Category: Tuchman, M.]]
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[[Category: Yu, X.]]
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[[Category: Amino-acid biosynthesis]]
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[[Category: Arginine biosynthesis]]
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[[Category: Cytoplasm]]
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[[Category: Transcarbamylase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 13:32:44 2010''
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Current revision

Crystal structure of acetylornithine transcarbamylase complexed with acetylcitrulline

PDB ID 3kzk

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