3m4g

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{{STRUCTURE_3m4g| PDB=3m4g | SCENE= }}
 
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===H57A HFQ from Pseudomonas Aeruginosa===
 
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{{ABSTRACT_PUBMED_20606268}}
 
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==Function==
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==H57A HFQ from Pseudomonas Aeruginosa==
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[[http://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
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<StructureSection load='3m4g' size='340' side='right'caption='[[3m4g]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3m4g]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M4G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4g OCA], [https://pdbe.org/3m4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m4g RCSB], [https://www.ebi.ac.uk/pdbsum/3m4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m4g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/3m4g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m4g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The bacterial Sm-like protein Hfq forms homohexamers both in solution and in crystals. The monomers are organized as a continuous beta-sheet passing through the whole hexamer ring with a common hydrophobic core. Analysis of the Pseudomonas aeruginosa Hfq (PaeHfq) hexamer structure suggested that solvent-inaccessible intermonomer hydrogen bonds created by conserved amino-acid residues should also stabilize the quaternary structure of the protein. In this work, one such conserved residue, His57, in PaeHfq was replaced by alanine, threonine or asparagine. The crystal structures of His57Thr and His57Ala Hfq were determined and the stabilities of all of the mutant forms and of the wild-type protein were measured. The results obtained demonstrate the great importance of solvent-inaccessible conserved hydrogen bonds between the Hfq monomers in stabilization of the hexamer structure.
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==About this Structure==
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The structures of mutant forms of Hfq from Pseudomonas aeruginosa reveal the importance of the conserved His57 for the protein hexamer organization.,Moskaleva O, Melnik B, Gabdulkhakov A, Garber M, Nikonov S, Stolboushkina E, Nikulin A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jul 1;66(Pt, 7):760-4. Epub 2010 Jun 23. PMID:20606268<ref>PMID:20606268</ref>
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[[3m4g]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4G OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020606268</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 3m4g" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Protein Hfq|Protein Hfq]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Gabdulkhakov, A.]]
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[[Category: Gabdulkhakov A]]
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[[Category: Garber, M.]]
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[[Category: Garber M]]
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[[Category: Melnik, B.]]
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[[Category: Melnik B]]
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[[Category: Moskaleva, O.]]
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[[Category: Moskaleva O]]
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[[Category: Nikonov, S.]]
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[[Category: Nikonov S]]
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[[Category: Nikulin, A.]]
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[[Category: Nikulin A]]
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[[Category: Stolboushkina, E.]]
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[[Category: Stolboushkina E]]
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[[Category: Hfq]]
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[[Category: Protein stability]]
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[[Category: Protein tertiary structure]]
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[[Category: Rna binding protein]]
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[[Category: Rna-binding]]
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[[Category: Stress response]]
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Current revision

H57A HFQ from Pseudomonas Aeruginosa

PDB ID 3m4g

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