3mjs

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{{Seed}}
 
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[[Image:3mjs.jpg|left|200px]]
 
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==Structure of A-type Ketoreductases from Modular Polyketide Synthase==
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The line below this paragraph, containing "STRUCTURE_3mjs", creates the "Structure Box" on the page.
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<StructureSection load='3mjs' size='340' side='right'caption='[[3mjs]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3mjs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_nodosus Streptomyces nodosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MJS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
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{{STRUCTURE_3mjs| PDB=3mjs | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjs OCA], [https://pdbe.org/3mjs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mjs RCSB], [https://www.ebi.ac.uk/pdbsum/3mjs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93NW7_9ACTN Q93NW7_9ACTN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/3mjs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mjs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Complex polyketides are characterized by multiple chiral centers harboring hydroxyl and alkyl substituents. To investigate the mechanisms by which these stereocenters are set, several high-resolution structures of the ketoreductase (KR) domain from the second module of the amphotericin modular polyketide synthase (PKS) were solved. This first structural analysis of an A-type KR helps reveal how these KRs direct polyketide intermediates into their active sites from the side opposite that used by B-type KRs, resulting in a beta-hydroxyl group of opposite stereochemistry. A comparison of structures obtained in the absence and presence of ligands reveals an induced fit mechanism that is important for catalysis. Activity assays of mutants of KRs from the first and second modules of the amphotericin PKS reveal the relative contributions of several active site residues toward catalysis and stereocontrol. Together, these results highlight the possibility of region-specific modification of polyketides through active site engineering of KRs.
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===Structure of A-type Ketoreductases from Modular Polyketide Synthase===
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Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase.,Zheng J, Taylor CA, Piasecki SK, Keatinge-Clay AT Structure. 2010 Aug 11;18(8):913-22. PMID:20696392<ref>PMID:20696392</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20696392}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3mjs" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20696392 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20696392}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3MJS is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Streptomyces_nodosus Streptomyces nodosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJS OCA].
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==Reference==
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<ref group="xtra">PMID:20696392</ref><references group="xtra"/>
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[[Category: Streptomyces nodosus]]
[[Category: Streptomyces nodosus]]
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[[Category: Keatinge-Clay, A T.]]
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[[Category: Keatinge-Clay AT]]
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[[Category: Piasecki, S K.]]
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[[Category: Piasecki SK]]
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[[Category: Taylor, C A.]]
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[[Category: Taylor CA]]
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[[Category: Zheng, J.]]
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[[Category: Zheng J]]
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[[Category: Oxidoreductase]]
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[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:45:37 2010''
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Current revision

Structure of A-type Ketoreductases from Modular Polyketide Synthase

PDB ID 3mjs

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