This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3n7l
From Proteopedia
(Difference between revisions)
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | {{STRUCTURE_3n7l| PDB=3n7l | SCENE= }} | ||
| - | ===Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain=== | ||
| - | {{ABSTRACT_PUBMED_20731382}} | ||
| - | == | + | ==Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain== |
| - | [[3n7l]] is a 1 chain structure with sequence from [ | + | <StructureSection load='3n7l' size='340' side='right'caption='[[3n7l]], [[Resolution|resolution]] 2.00Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3n7l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N7L FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7l OCA], [https://pdbe.org/3n7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n7l RCSB], [https://www.ebi.ac.uk/pdbsum/3n7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n7l ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9LBR1_CLOBO Q9LBR1_CLOBO] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/3n7l_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n7l ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The botulinum neurotoxins (BoNTs) are the most potent protein toxins for humans. There are seven serotypes of BoNTs (A-G) based on a lack of cross antiserum neutralization. BoNTs utilize gangliosides as components of the host receptors for binding and entry into neurons. Members of BoNT/C and BoNT/D serotypes include mosaic toxins that are organized in D/C and C/D toxins. One D/C mosaic toxin, BoNT/D-South Africa (BoNT/D-SA), was not fully neutralized by immunization with BoNT serotype C or D, which stimulated this study. Here the crystal structures of the receptor binding domains of BoNT/C, BoNT/D, and BoNT/D-SA are presented. Biochemical and cell binding studies show that BoNT/C and BoNT/D-SA possess unique mechanisms for ganglioside binding. These studies provide new information about how the BoNTs can enter host cells as well as a basis for understanding the immunological diversity of these neurotoxins. | ||
| - | + | Identification of a Unique Ganglioside Binding Loop within Botulinum Neurotoxins C and D-SA .,Karalewitz AP, Kroken AR, Fu Z, Baldwin MR, Kim JJ, Barbieri JT Biochemistry. 2010 Aug 23. PMID:20731382<ref>PMID:20731382</ref> | |
| - | <ref | + | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3n7l" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Clostridium botulinum]] | [[Category: Clostridium botulinum]] | ||
| - | [[Category: Baldwin | + | [[Category: Large Structures]] |
| - | [[Category: Barbieri | + | [[Category: Baldwin MR]] |
| - | [[Category: Fu | + | [[Category: Barbieri JT]] |
| - | [[Category: Karalewitz | + | [[Category: Fu Z]] |
| - | [[Category: Kim | + | [[Category: Karalewitz A]] |
| - | [[Category: Kroken | + | [[Category: Kim J-JP]] |
| - | + | [[Category: Kroken A]] | |
| - | + | ||
| - | + | ||
| - | + | ||
Current revision
Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain
| |||||||||||
