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3ns0

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[[Image:3ns0.png|left|200px]]
 
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==X-ray structure of bacteriorhodopsin==
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The line below this paragraph, containing "STRUCTURE_3ns0", creates the "Structure Box" on the page.
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<StructureSection load='3ns0' size='340' side='right'caption='[[3ns0]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ns0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NS0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
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{{STRUCTURE_3ns0| PDB=3ns0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ns0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ns0 OCA], [https://pdbe.org/3ns0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ns0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ns0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ns0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriorhodopsin (bR) provides light-driven vectorial proton transport across a cell membrane. Creation of electrochemical potential at the membrane is a universal step in energy transformation in a cell. Published atomic crystallographic models of early intermediate states of bR show a significant difference between them, and conclusions about pumping mechanisms have been contradictory. Here, we present a quantitative high-resolution crystallographic study of conformational changes in bR induced by X-ray absorption. It is shown that X-ray doses that are usually accumulated during data collection for intermediate-state studies are sufficient to significantly alter the structure of the protein. X-ray-induced changes occur primarily in the active site of bR. Structural modeling showed that X-ray absorption triggers retinal isomerization accompanied by the disappearance of electron densities corresponding to the water molecule W402 bound to the Schiff base. It is demonstrated that these and other X-ray-induced changes may mimic functional conformational changes of bR leading to misinterpretation of the earlier obtained X-ray crystallographic structures of photointermediates.
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===X-ray structure of bacteriorhodopsin===
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X-ray-Radiation-Induced Changes in Bacteriorhodopsin Structure.,Borshchevskiy VI, Round ES, Popov AN, Buldt G, Gordeliy VI J Mol Biol. 2011 Apr 21. PMID:21530535<ref>PMID:21530535</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ns0" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21530535}}, adds the Publication Abstract to the page
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*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21530535 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21530535}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3ns0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NS0 OCA].
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==Reference==
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<ref group="xtra">PMID:021530535</ref><references group="xtra"/>
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[[Category: Halobacterium salinarum]]
[[Category: Halobacterium salinarum]]
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[[Category: Borshchevskiy, V I.]]
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[[Category: Large Structures]]
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[[Category: Borshchevskiy VI]]

Current revision

X-ray structure of bacteriorhodopsin

PDB ID 3ns0

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