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3nz8

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Crystal structure of the HIV-2 neutralizing Fab fragment 7C8

Structural highlights

3nz8 is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

7C8 is a mouse monoclonal antibody specific for the third hypervariable region (V3) of the human immunodeficiency virus type 2 (HIV-2)-associated protein gp125. The three-dimensional crystal structure of the Fab fragment of 7C8, determined to 2.7 A resolution, reveals a deep and narrow antigen-binding cleft with architecture appropriate for an elongated epitope. The highly hydrophobic cleft is bordered on one side by the negatively charged second complementarity determining region (CDR2) and the unusually long positively charged CDR3 of the heavy chain and, on the other side, by the CDR1 of the light chain. Analysis of 7C8 in complex with molecular models of monomeric and trimeric gp125 highlights the importance of a conserved stretch of residues FHSQ that is localized centrally on the V3 region of gp125. Furthermore, modeling also indicates that the Fab fragment neutralizes the virus by sterically impairing subsequent engagement of the gp125 trimer with the co-receptor on the target cell.

Crystal Structure of the HIV-2 Neutralizing Fab Fragment 7C8 with High Specificity to the V3 Region of gp125.,Uchtenhagen H, Friemann R, Raszewski G, Spetz AL, Nilsson L, Achour A PLoS One. 2011 Apr 26;6(4):e18767. PMID:21541316^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Uchtenhagen H, Friemann R, Raszewski G, Spetz AL, Nilsson L, Achour A. Crystal Structure of the HIV-2 Neutralizing Fab Fragment 7C8 with High Specificity to the V3 Region of gp125. PLoS One. 2011 Apr 26;6(4):e18767. PMID:21541316 doi:10.1371/journal.pone.0018767

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nz8"

@@ Line 3: / Line 3: @@
 <StructureSection load='3nz8' size='340' side='right'caption='[[3nz8]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nz8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NZ8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nz8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NZ8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nz8 OCA], [http://pdbe.org/3nz8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nz8 RCSB], [http://www.ebi.ac.uk/pdbsum/3nz8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nz8 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nz8 OCA], [https://pdbe.org/3nz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nz8 RCSB], [https://www.ebi.ac.uk/pdbsum/3nz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nz8 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 20: @@
 ==See Also==
 *[[Antibody 3D structures|Antibody 3D structures]]
+*[[Sandbox 20009|Sandbox 20009]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 == References ==
 <references/>
@@ Line 24: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Achour, A]]
+[[Category: Achour A]]
-[[Category: Friemann, R]]
+[[Category: Friemann R]]
-[[Category: Nilsson, L]]
+[[Category: Nilsson L]]
-[[Category: Raszewski, G]]
+[[Category: Raszewski G]]
-[[Category: Spetz, A L]]
+[[Category: Spetz A-L]]
-[[Category: Uchtenhagen, H]]
+[[Category: Uchtenhagen H]]
-[[Category: Antibody]]
-[[Category: Antigen binding]]
-[[Category: Fab]]
-[[Category: Gp125]]
-[[Category: Hiv-2]]
-[[Category: Immune system]]
-[[Category: Immunological]]
-[[Category: V3]]

3nz8

From Proteopedia

Current revision

Crystal structure of the HIV-2 neutralizing Fab fragment 7C8

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

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