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3ofj

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Crystal structure of N-methyltransferase NodS from Bradyrhizobium japonicum WM9

Structural highlights

3ofj is a 1 chain structure with sequence from Bradyrhizobium sp. WM9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.43Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9AQ22_BRASW SAM-utilizing methyltransferase involved in nod factor synthesis.[PIRNR:PIRNR009310]

Publication Abstract from PubMed

NodS is an S-adenosyl-l-methionine (SAM)-dependent N-methyltransferase that is involved in the biosynthesis of Nod factor (NF) in rhizobia, which are bacterial symbionts of legume plants. NF is a modified chitooligosaccharide (COS) signal molecule that is recognized by the legume host, where it initiates symbiotic processes leading to atmospheric nitrogen fixation. We report the crystal structure of recombinant NodS protein from Bradyrhizobium japonicum, which infects lupine and serradella legumes. Two crystal forms-ligand-free NodS and NodS in complex with S-adenosyl-l-homocysteine, which is a by-product of the methylation reaction-were obtained, and their structures were refined to resolutions of 2.43 A and 1.85 A, respectively. Although the overall fold (consisting of a seven-stranded beta-sheet flanked by layers of helices) is similar to those of other SAM-dependent methyltransferases, NodS has specific features reflecting the unique character of its oligosaccharide substrate. In particular, the N-terminal helix and its connecting loop get ordered upon SAM binding, thereby closing the methyl donor cavity and shaping a long surface canyon that is clearly the binding site for the acceptor molecule. Comparison of the two structural forms of NodS suggests that there are also other conformational changes taking place upon the binding of the donor substrate. As an enzyme that methylates a COS substrate, NodS is the first example among all SAM-dependent methyltransferases to have its three-dimensional structure elucidated. Gaining insight about how NodS binds its donor and acceptor substrates helps to better understand the mechanism of NodS activity and the basis of its functional difference in various rhizobia.

Crystal Structures of NodS N-Methyltransferase from Bradyrhizobium japonicum in Ligand-Free Form and as SAH Complex.,Cakici O, Sikorski M, Stepkowski T, Bujacz G, Jaskolski M J Mol Biol. 2010 Oct 21. PMID:20970431^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cakici O, Sikorski M, Stepkowski T, Bujacz G, Jaskolski M. Crystal Structures of NodS N-Methyltransferase from Bradyrhizobium japonicum in Ligand-Free Form and as SAH Complex. J Mol Biol. 2010 Oct 21. PMID:20970431 doi:10.1016/j.jmb.2010.10.016

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ofj"

@@ Line 3: / Line 3: @@
 <StructureSection load='3ofj' size='340' side='right'caption='[[3ofj]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ofj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brasw Brasw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OFJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ofj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_sp._WM9 Bradyrhizobium sp. WM9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OFJ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ofk|3ofk]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nodS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=133505 BRASW])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ofj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ofj OCA], [https://pdbe.org/3ofj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ofj RCSB], [https://www.ebi.ac.uk/pdbsum/3ofj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ofj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/Q9AQ22_BRASW Q9AQ22_BRASW]] SAM-utilizing methyltransferase involved in nod factor synthesis.[PIRNR:PIRNR009310]
+[https://www.uniprot.org/uniprot/Q9AQ22_BRASW Q9AQ22_BRASW] SAM-utilizing methyltransferase involved in nod factor synthesis.[PIRNR:PIRNR009310]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Brasw]]
+[[Category: Bradyrhizobium sp. WM9]]
 [[Category: Large Structures]]
-[[Category: Bujacz, G]]
+[[Category: Bujacz G]]
-[[Category: Cakici, O]]
+[[Category: Cakici O]]
-[[Category: Jaskolski, M]]
+[[Category: Jaskolski M]]
-[[Category: Sikorski, M]]
+[[Category: Sikorski M]]
-[[Category: Stepkowski, T]]
+[[Category: Stepkowski T]]
-[[Category: Alpha/beta structure]]
-[[Category: Lipochitooligosaccharide]]
-[[Category: Methylation]]
-[[Category: N-methyltransferase]]
-[[Category: Nitrogen fixation]]
-[[Category: Nod]]
-[[Category: Nod factor]]
-[[Category: Nodulation]]
-[[Category: Sah]]
-[[Category: Sam]]
-[[Category: Sam-dependent n-methyltransferase]]
-[[Category: Symbiosis]]
-[[Category: Transferase]]
-[[Category: Variant of rossmann fold]]

3ofj

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Current revision

Crystal structure of N-methyltransferase NodS from Bradyrhizobium japonicum WM9

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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