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| | ==Crystal structure of the Arabidopsis receptor kinase HAESA in complex with the peptide hormone IDA and the co-receptor SERK1== | | ==Crystal structure of the Arabidopsis receptor kinase HAESA in complex with the peptide hormone IDA and the co-receptor SERK1== |
| - | <StructureSection load='5iyx' size='340' side='right' caption='[[5iyx]], [[Resolution|resolution]] 2.43Å' scene=''> | + | <StructureSection load='5iyx' size='340' side='right'caption='[[5iyx]], [[Resolution|resolution]] 2.43Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5iyx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IYX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5iyx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IYX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RLK5, HAE, At4g28490, F21O9.180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), IDA, At1g68765, F14K14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), SERK1, At1g71830, F14O23.21, F14O23_24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iyx OCA], [https://pdbe.org/5iyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iyx RCSB], [https://www.ebi.ac.uk/pdbsum/5iyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iyx ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iyx OCA], [http://pdbe.org/5iyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iyx RCSB], [http://www.ebi.ac.uk/pdbsum/5iyx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iyx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RLK5_ARATH RLK5_ARATH]] Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.<ref>PMID:10640280</ref> <ref>PMID:18660431</ref> <ref>PMID:18809915</ref> [[http://www.uniprot.org/uniprot/SERK1_ARATH SERK1_ARATH]] Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Phosphorylates BRI1 on 'Ser-887' and CDC48 on at least one threonine residue and on 'Ser-41'. Confers embryogenic competence. Acts redundantly with SERK2 as a control point for sporophytic development controlling male gametophyte production. Involved in the brassinolide signaling pathway.<ref>PMID:11509554</ref> <ref>PMID:17693538</ref> [[http://www.uniprot.org/uniprot/IDA_ARATH IDA_ARATH]] Involved in an ethylene-independent separation step of floral abscission. May act with RLK5 and HSL2 as ligand-receptor pairs.<ref>PMID:18660431</ref> | + | [https://www.uniprot.org/uniprot/RLK5_ARATH RLK5_ARATH] Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.<ref>PMID:10640280</ref> <ref>PMID:18660431</ref> <ref>PMID:18809915</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Hothorn, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Santiago, J]] | + | [[Category: Hothorn M]] |
| - | [[Category: Membrane receptor kinase]] | + | [[Category: Santiago J]] |
| - | [[Category: Organ shedding]]
| + | |
| - | [[Category: Peptide hormone receptor]]
| + | |
| - | [[Category: Plant development]]
| + | |
| - | [[Category: Signaling complex]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
RLK5_ARATH Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.[1] [2] [3]
Publication Abstract from PubMed
Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.
Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.,Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M Elife. 2016 Apr 8;5. pii: e15075. doi: 10.7554/eLife.15075. PMID:27058169[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jinn TL, Stone JM, Walker JC. HAESA, an Arabidopsis leucine-rich repeat receptor kinase, controls floral organ abscission. Genes Dev. 2000 Jan 1;14(1):108-17. PMID:10640280
- ↑ Stenvik GE, Tandstad NM, Guo Y, Shi CL, Kristiansen W, Holmgren A, Clark SE, Aalen RB, Butenko MA. The EPIP peptide of INFLORESCENCE DEFICIENT IN ABSCISSION is sufficient to induce abscission in arabidopsis through the receptor-like kinases HAESA and HAESA-LIKE2. Plant Cell. 2008 Jul;20(7):1805-17. doi: 10.1105/tpc.108.059139. Epub 2008 Jul, 25. PMID:18660431 doi:http://dx.doi.org/10.1105/tpc.108.059139
- ↑ Cho SK, Larue CT, Chevalier D, Wang H, Jinn TL, Zhang S, Walker JC. Regulation of floral organ abscission in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15629-34. doi:, 10.1073/pnas.0805539105. Epub 2008 Sep 22. PMID:18809915 doi:http://dx.doi.org/10.1073/pnas.0805539105
- ↑ Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M. Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. Elife. 2016 Apr 8;5. pii: e15075. doi: 10.7554/eLife.15075. PMID:27058169 doi:http://dx.doi.org/10.7554/eLife.15075
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