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5j45

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'''Unreleased structure'''
 
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The entry 5j45 is ON HOLD until Paper Publication
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==Crystal structure of Shrub, fly ortholog of SNF7/CHMP4B==
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<StructureSection load='5j45' size='340' side='right'caption='[[5j45]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5j45]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J45 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.758&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j45 OCA], [https://pdbe.org/5j45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j45 RCSB], [https://www.ebi.ac.uk/pdbsum/5j45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j45 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8T0Q4_DROME Q8T0Q4_DROME]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The endosomal sorting complex required for transport (ESCRT) is a conserved protein complex that facilitates budding and fission of membranes. It executes a key step in many cellular events, including cytokinesis and multi-vesicular body formation. The ESCRT-III protein Shrub in flies, or its homologs in yeast (Snf7) or humans (CHMP4B), is a critical polymerizing component of ESCRT-III needed to effect membrane fission. We report the structural basis for polymerization of Shrub and define a minimal region required for filament formation. The X-ray structure of the Shrub core shows that individual monomers in the lattice interact in a staggered arrangement using complementary electrostatic surfaces. Mutations that disrupt interface salt bridges interfere with Shrub polymerization and function. Despite substantial sequence divergence and differences in packing interactions, the arrangement of Shrub subunits in the polymer resembles that of Snf7 and other family homologs, suggesting that this intermolecular packing mechanism is shared among ESCRT-III proteins.
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Authors: McMillan, B.J., Blacklow, S.C.
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Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein.,McMillan BJ, Tibbe C, Jeon H, Drabek AA, Klein T, Blacklow SC Cell Rep. 2016 Aug 2;16(5):1211-7. doi: 10.1016/j.celrep.2016.06.093. Epub 2016, Jul 21. PMID:27452459<ref>PMID:27452459</ref>
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Description: Crystal structure of Shrub, fly ortholog of SNF7/CHMP4B
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Mcmillan, B.J]]
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<div class="pdbe-citations 5j45" style="background-color:#fffaf0;"></div>
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[[Category: Blacklow, S.C]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Drosophila melanogaster]]
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[[Category: Large Structures]]
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[[Category: Blacklow SC]]
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[[Category: McMillan BJ]]

Current revision

Crystal structure of Shrub, fly ortholog of SNF7/CHMP4B

PDB ID 5j45

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