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3rja
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==Crystal structure of carbohydrate oxidase from Microdochium nivale in complex with substrate analogue== | ==Crystal structure of carbohydrate oxidase from Microdochium nivale in complex with substrate analogue== | ||
| - | <StructureSection load='3rja' size='340' side='right' caption='[[3rja]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3rja' size='340' side='right'caption='[[3rja]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3rja]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3rja]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microdochium_nivale Microdochium nivale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RJA FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABL:(2R,3R,4R,5R)-4,5-DIHYDROXY-2-(HYDROXYMETHYL)-6-OXOPIPERIDIN-3-YL+BETA-D-GLUCOPYRANOSIDE'>ABL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABL:(2R,3R,4R,5R)-4,5-DIHYDROXY-2-(HYDROXYMETHYL)-6-OXOPIPERIDIN-3-YL+BETA-D-GLUCOPYRANOSIDE'>ABL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rja OCA], [https://pdbe.org/3rja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rja RCSB], [https://www.ebi.ac.uk/pdbsum/3rja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rja ProSAT]</span></td></tr> | |
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| - | + | == Function == | |
| - | = | + | [https://www.uniprot.org/uniprot/MNCO_MICNN MNCO_MICNN] Catalyzes the selective oxidation of C1 hydroxyl moieties on mono-, oligo- and polysaccharides with concomitant reduction of molecular oxygen to hydrogen peroxide. This results in the formation of the corresponding lactones, which typically undergo spontaneous hydrolysis. Carbohydrate oxidase is able to oxidize a variety of substrates including D-glucose, D-galactose, D-xylose, D-maltose, D-cellobiose, and lactose. In addition, among various oligosaccharides, the enzyme preferred tetrameric dextrins, indicating a favorable interaction of four linked glucose units with the substrate binding pocket.<ref>PMID:11179980</ref> [PROSITE-ProRule:PRU00718]<ref>PMID:11179980</ref> |
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| - | </ | + | |
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
[[Category: Microdochium nivale]] | [[Category: Microdochium nivale]] | ||
| - | [[Category: Dohnalek | + | [[Category: Dohnalek J]] |
| - | [[Category: Duskova | + | [[Category: Duskova J]] |
| - | [[Category: Fuglsang | + | [[Category: Fuglsang CC]] |
| - | [[Category: Hasek | + | [[Category: Hasek J]] |
| - | [[Category: Kolenko | + | [[Category: Kolenko P]] |
| - | [[Category: Koval | + | [[Category: Koval T]] |
| - | [[Category: Ostergaard | + | [[Category: Ostergaard LH]] |
| - | [[Category: Skalova | + | [[Category: Skalova T]] |
| - | [[Category: Stepankova | + | [[Category: Stepankova A]] |
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Current revision
Crystal structure of carbohydrate oxidase from Microdochium nivale in complex with substrate analogue
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