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4hkr

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Calcium release-activated calcium (CRAC) channel ORAI

Structural highlights

4hkr is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3511Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRCM1_DROME Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.^[1] ^[2]

Publication Abstract from PubMed

The plasma membrane protein Orai forms the pore of the calcium release-activated calcium (CRAC) channel and generates sustained cytosolic calcium signals when triggered by depletion of calcium from the endoplasmic reticulum. The crystal structure of Orai from Drosophila melanogaster, determined at 3.35 angstrom resolution, reveals that the calcium channel is composed of a hexameric assembly of Orai subunits arranged around a central ion pore. The pore traverses the membrane and extends into the cytosol. A ring of glutamate residues on its extracellular side forms the selectivity filter. A basic region near the intracellular side can bind anions that may stabilize the closed state. The architecture of the channel differs markedly from other ion channels and gives insight into the principles of selective calcium permeation and gating.

Crystal structure of the calcium release-activated calcium channel Orai.,Hou X, Pedi L, Diver MM, Long SB Science. 2012 Dec 7;338(6112):1308-13. doi: 10.1126/science.1228757. Epub 2012, Nov 22. PMID:23180775^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Feske S, Gwack Y, Prakriya M, Srikanth S, Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 2006 May 11;441(7090):179-85. Epub 2006 Apr 2. PMID:16582901 doi:http://dx.doi.org/nature04702
↑ Vig M, Peinelt C, Beck A, Koomoa DL, Rabah D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R, Kinet JP. CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science. 2006 May 26;312(5777):1220-3. Epub 2006 Apr 27. PMID:16645049 doi:http://dx.doi.org/10.1126/science.1127883
↑ Hou X, Pedi L, Diver MM, Long SB. Crystal structure of the calcium release-activated calcium channel Orai. Science. 2012 Dec 7;338(6112):1308-13. doi: 10.1126/science.1228757. Epub 2012, Nov 22. PMID:23180775 doi:http://dx.doi.org/10.1126/science.1228757

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4hkr"

Categories: Drosophila melanogaster | Large Structures | Hou X | Long SB

@@ Line 1: / Line 1: @@
 ==Calcium release-activated calcium (CRAC) channel ORAI==
-<StructureSection load='4hkr' size='340' side='right' caption='[[4hkr]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
+<StructureSection load='4hkr' size='340' side='right'caption='[[4hkr]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4hkr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HKR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HKR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4hkr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HKR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3511&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">olf186-F, CRACM1, olf186, CG11430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hkr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hkr RCSB], [http://www.ebi.ac.uk/pdbsum/4hkr PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hkr OCA], [https://pdbe.org/4hkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hkr RCSB], [https://www.ebi.ac.uk/pdbsum/4hkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hkr ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME]] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref>
+[https://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4hkr" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Hou, X]]
+[[Category: Large Structures]]
-[[Category: Long, S B]]
+[[Category: Hou X]]
-[[Category: Calcium channel]]
+[[Category: Long SB]]
-[[Category: Eukaryotic membrane protein]]
-[[Category: Ion channel]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Orai1]]
-[[Category: Stim]]
-[[Category: Transport protein]]

4hkr

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Current revision

Calcium release-activated calcium (CRAC) channel ORAI

Structural highlights

Function

Publication Abstract from PubMed

References

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