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4hr7

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{{STRUCTURE_4hr7| PDB=4hr7 | SCENE= }}
 
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===Crystal Structure of Biotin Carboxyl Carrier Protein-Biotin Carboxylase Complex from E.coli===
 
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==About this Structure==
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==Crystal Structure of Biotin Carboxyl Carrier Protein-Biotin Carboxylase Complex from E.coli==
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[[4hr7]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HR7 OCA].
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<StructureSection load='4hr7' size='340' side='right'caption='[[4hr7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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[[Category: Escherichia coli k-12]]
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== Structural highlights ==
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[[Category: Broussard, T C.]]
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<table><tr><td colspan='2'>[[4hr7]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HR7 FirstGlance]. <br>
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[[Category: Champion, T S.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.495&#8491;</td></tr>
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[[Category: Kobe, M J.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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[[Category: Neau, D B.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hr7 OCA], [https://pdbe.org/4hr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hr7 RCSB], [https://www.ebi.ac.uk/pdbsum/4hr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hr7 ProSAT]</span></td></tr>
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[[Category: Pakhomova, S.]]
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</table>
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[[Category: Price, A E.]]
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== Function ==
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[[Category: Waldrop, G L.]]
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[https://www.uniprot.org/uniprot/ACCC_ECOLI ACCC_ECOLI] This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.
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[[Category: Acetyl-coa carboxylase]]
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<div style="background-color:#fffaf0;">
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[[Category: Antibiotic target]]
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== Publication Abstract from PubMed ==
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[[Category: Atp grasp]]
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Acetyl-coenzyme A (acetyl-CoA) carboxylase is a biotin-dependent, multifunctional enzyme that catalyzes the regulated step in fatty acid synthesis. The Escherichia coli enzyme is composed of a homodimeric biotin carboxylase (BC), biotinylated biotin carboxyl carrier protein (BCCP), and an alpha2beta2 heterotetrameric carboxyltransferase. This enzyme complex catalyzes two half-reactions to form malonyl-coenzyme A. BC and BCCP participate in the first half-reaction, whereas carboxyltransferase and BCCP are involved in the second. Three-dimensional structures have been reported for the individual subunits; however, the structural basis for how BCCP reacts with the carboxylase or transferase is unknown. Therefore, we report here the crystal structure of E. coli BCCP complexed with BC to a resolution of 2.49 A. The protein-protein complex shows a unique quaternary structure and two distinct interfaces for each BCCP monomer. These BCCP binding sites are unique compared to phylogenetically related biotin-dependent carboxylases and therefore provide novel targets for developing antibiotics against bacterial acetyl-CoA carboxylase.
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[[Category: Biotin carboxyl carrier protein]]
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[[Category: Biotin carboxylase]]
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The Three-Dimensional Structure of the Biotin Carboxylase-Biotin Carboxyl Carrier Protein Complex of E. coli Acetyl-CoA Carboxylase.,Broussard TC, Kobe MJ, Pakhomova S, Neau DB, Price AE, Champion TS, Waldrop GL Structure. 2013 Mar 12. pii: S0969-2126(13)00041-5. doi:, 10.1016/j.str.2013.02.001. PMID:23499019<ref>PMID:23499019</ref>
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[[Category: Biotin-dependent carboxylase]]
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[[Category: Fatty acid synthesis]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Ligase-biotin binding protein complex]]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 4hr7" style="background-color:#fffaf0;"></div>
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[[Category: Protein interface]]
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[[Category: Protein-protein interaction]]
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==See Also==
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*[[Acetyl-CoA carboxylase 3D structures|Acetyl-CoA carboxylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Broussard TC]]
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[[Category: Champion TS]]
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[[Category: Kobe MJ]]
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[[Category: Neau DB]]
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[[Category: Pakhomova S]]
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[[Category: Price AE]]
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[[Category: Waldrop GL]]

Current revision

Crystal Structure of Biotin Carboxyl Carrier Protein-Biotin Carboxylase Complex from E.coli

PDB ID 4hr7

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