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4ip6

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{{STRUCTURE_4ip6| PDB=4ip6 | SCENE= }}
 
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===C-terminal domain of the thiol:disulfide interchange protein DsbD, Q488A mutant===
 
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==Function==
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==C-terminal domain of the thiol:disulfide interchange protein DsbD, Q488A mutant==
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[[http://www.uniprot.org/uniprot/DSBD_ECOLI DSBD_ECOLI]] Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm, thereby maintaining the active site of DsbC, DsbE and DsbG in a reduced state. This transfer involves a cascade of disulfide bond formation and reduction steps.[HAMAP-Rule:MF_00399]
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<StructureSection load='4ip6' size='340' side='right'caption='[[4ip6]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ip6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IP6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IP6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ip6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ip6 OCA], [https://pdbe.org/4ip6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ip6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ip6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ip6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DSBD_ECOLI DSBD_ECOLI] Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm, thereby maintaining the active site of DsbC, DsbE and DsbG in a reduced state. This transfer involves a cascade of disulfide bond formation and reduction steps.[HAMAP-Rule:MF_00399]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proteins belonging to the thioredoxin superfamily are abundant in all organisms. They share the same structural features, arranged in a seemingly simple fold, but they perform a multitude of functions in oxidative protein folding and electron transfer pathways. We use the C-terminal domain of the unique transmembrane reductant conductor DsbD (cDsbD) as a model for an in-depth analysis of the factors controlling the reactivity of the Trx fold. We employ NMR spectroscopy, X-ray crystallography, mutagenesis, in vivo functional experiments applied to DsbD and a comparative sequence analysis of Trx-fold proteins to determine the effect of residues in the vicinity of the active site on the ionization of the key nucleophilic cysteine of the -CXXC- motif. We show that the function and reactivity of Trx-fold proteins depend critically on the electrostatic features imposed by an extended active-site motif.
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==About this Structure==
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An Extended Active-site Motif Controls the Reactivity of the Thioredoxin Fold.,Mavridou DA, Saridakis E, Kritsiligkou P, Mozley EC, Ferguson SJ, Redfield C J Biol Chem. 2014 Jan 27. PMID:24469455<ref>PMID:24469455</ref>
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[[4ip6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IP6 OCA].
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[[Category: Protein-disulfide reductase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Mavridou, D A.I.]]
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</div>
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[[Category: Redfield, C.]]
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<div class="pdbe-citations 4ip6" style="background-color:#fffaf0;"></div>
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[[Category: Saridakis, E.]]
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[[Category: Bacterial periplasm]]
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==See Also==
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[[Category: Oxidoreductase]]
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*[[Thiol:disulfide interchange protein 3D structures|Thiol:disulfide interchange protein 3D structures]]
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[[Category: Thiol:disulfide oxidoreductase]]
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== References ==
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[[Category: Thioredoxin]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Mavridou DAI]]
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[[Category: Redfield C]]
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[[Category: Saridakis E]]

Current revision

C-terminal domain of the thiol:disulfide interchange protein DsbD, Q488A mutant

PDB ID 4ip6

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