4iug
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| - | {{STRUCTURE_4iug| PDB=4iug | SCENE= }} | ||
| - | ===Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose=== | ||
| - | {{ABSTRACT_PUBMED_23688418}} | ||
| - | == | + | ==Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose== |
| - | [[http://www.uniprot.org/uniprot/BGALA_ASPOR BGALA_ASPOR | + | <StructureSection load='4iug' size='340' side='right'caption='[[4iug]], [[Resolution|resolution]] 2.60Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4iug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IUG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=M6D:6-O-PHOSPHONO-BETA-D-MANNOPYRANOSE'>M6D</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iug OCA], [https://pdbe.org/4iug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iug RCSB], [https://www.ebi.ac.uk/pdbsum/4iug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iug ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BGALA_ASPOR BGALA_ASPOR] Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of the industrially important Aspergillus oryzae beta-galactosidase has been determined at 2.60A resolution. The Ao-beta-gal is a large (985 residues) monomeric multi-domain enzyme that has a catalytic (alpha/beta)8-barrel domain. An electron density map revealed extensive N-glycosylation between the domain interfaces suggesting that the oligosaccharide-chains would have a stabilizing role for the structure of Ao-beta-gal. Comparison of structure with other beta-galactosidase structures of glycoside hydrolase family 35 revealed a number of hydrophobic residues, which may contribute favorably to the stabilization of the structure. The role of a high number of acidic residues in Ao-beta-gal is also discussed. | ||
| - | + | The crystal structure of acidic beta-galactosidase from Aspergillus oryzae.,Maksimainen MM, Lampio A, Mertanen M, Turunen O, Rouvinen J Int J Biol Macromol. 2013 Sep;60:109-15. doi: 10.1016/j.ijbiomac.2013.05.003., Epub 2013 May 17. PMID:23688418<ref>PMID:23688418</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| + | <div class="pdbe-citations 4iug" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Galactosidase 3D structures|Galactosidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Aspergillus oryzae]] | [[Category: Aspergillus oryzae]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Maksimainen | + | [[Category: Maksimainen M]] |
| - | [[Category: Rouvinen | + | [[Category: Rouvinen J]] |
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| - | + | ||
Current revision
Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose
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