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4iye
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of AdTx1 (rho-Da1a) from eastern green mamba (Dendroaspis angusticeps)== | |
| + | <StructureSection load='4iye' size='340' side='right'caption='[[4iye]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4iye]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendroaspis_angusticeps Dendroaspis angusticeps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IYE FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.951Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iye OCA], [https://pdbe.org/4iye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iye RCSB], [https://www.ebi.ac.uk/pdbsum/4iye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iye ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/3SI1A_DENAN 3SI1A_DENAN] Orthosteric antagonist of the alpha-1A adrenergic receptor (ADRA1A) with an affinity of 0.35 nM (PubMed:20015090). Has also affinity for ADRA1B > ADRA2C > ADRA1D (PubMed:23935897). Acts as a relaxant of smooth muscle. Reduces prostatic muscle tone as efficiently as tamsulosin (an antagonist presently used), but with fewer cardiovascular side effects (PubMed:23005263).<ref>PMID:20015090</ref> <ref>PMID:21419153</ref> <ref>PMID:23005263</ref> <ref>PMID:23935897</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | rho-Da1a toxin from eastern green mamba (Dendroaspis angusticeps) venom is a polypeptide of 65 amino acids with a strong affinity for the G-protein-coupled alpha(1A)-adrenoceptor. This neurotoxin has been crystallized from resolubilized lyophilized powder, but the best crystals grew spontaneously during lyophilization. The crystals belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 37.37, c = 66.05 A, and diffracted to 1.95 A resolution. The structure solved by molecular replacement showed strong similarities to green mamba muscarinic toxins. | ||
| - | + | Crystallization of recombinant green mamba rho-Da1a toxin during a lyophilization procedure and its structure determination.,Maiga A, Vera L, Marchetti C, Lorphelin A, Bellanger L, Mourier G, Servent D, Gilles N, Stura EA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):704-9. doi:, 10.1107/S1744309113011470. Epub 2013 May 29. PMID:23722859<ref>PMID:23722859</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4iye" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Dendroaspis angusticeps]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bellanger L]] | ||
| + | [[Category: Gilles N]] | ||
| + | [[Category: Lorphelin A]] | ||
| + | [[Category: Maiga AA]] | ||
| + | [[Category: Marchetti C]] | ||
| + | [[Category: Servant D]] | ||
| + | [[Category: Stura EA]] | ||
| + | [[Category: Vera L]] | ||
Current revision
Crystal structure of AdTx1 (rho-Da1a) from eastern green mamba (Dendroaspis angusticeps)
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