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4k4b

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X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA

Structural highlights

4k4b is a 8 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MENI_ECOLI Catalyzes the hydrolysis of 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA) to 1,4-dihydroxy-2-naphthoate (DHNA). Also shows significant activity toward a wide range of acyl-CoA thioesters, and minimal activity toward benzoyl-holoEntB.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Herein, the structural determinants for substrate recognition and catalysis in two hotdog-fold thioesterase paralogs, YbdB and YdiI from Escherichia coli, are identified and analyzed to provide insight into the evolution of biological function in the hotdog-fold enzyme superfamily. The X-ray crystal structures of YbdB and YdiI, in complex with inert substrate analogs, determined in this study revealed the locations of the respective thioester substrate binding sites and the identity of the residues positioned for substrate binding and catalysis. The importance of each of these residues was assessed through amino acid replacements followed by steady-state kinetic analyses of the corresponding site-directed mutants. Transient kinetic and solvent (18)O-labeling studies were then carried out to provide insight into the role of Glu63 posited to function as the nucleophile or general base in catalysis. Finally, the structure-function-mechanism profiles of the two paralogs, along with that of a more distant homolog, were compared to identify conserved elements of substrate recognition and catalysis, which define the core traits of the hotdog-fold thioesterase family, as well as structural features that are unique to each thioesterase. Founded on the insight gained from this analysis, we conclude that the promiscuity revealed by in vitro substrate activity determinations, and posited to facilitate the evolution of new biological function, is the product of intrinsic plasticity in substrate binding as well as in the catalytic mechanism.

Structure and Catalysis in the Escherichia coli Hotdog-fold Thioesterase Paralogs YdiI and YbdB.,Wu R, Latham JA, Chen D, Farelli J, Zhao H, Matthews K, Allen KN, Dunaway-Mariano D Biochemistry. 2014 Jul 29;53(29):4788-805. doi: 10.1021/bi500334v. Epub 2014 Jul , 18. PMID:25010423^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kuznetsova E, Proudfoot M, Sanders SA, Reinking J, Savchenko A, Arrowsmith CH, Edwards AM, Yakunin AF. Enzyme genomics: Application of general enzymatic screens to discover new enzymes. FEMS Microbiol Rev. 2005 Apr;29(2):263-79. PMID:15808744 doi:S0168-6445(05)00004-5
↑ Chen M, Ma X, Chen X, Jiang M, Song H, Guo Z. Identification of a hotdog fold thioesterase involved in the biosynthesis of menaquinone in Escherichia coli. J Bacteriol. 2013 Jun;195(12):2768-75. doi: 10.1128/JB.00141-13. Epub 2013 Apr 5. PMID:23564174 doi:http://dx.doi.org/10.1128/JB.00141-13
↑ Latham JA, Chen D, Allen KN, Dunaway-Mariano D. Divergence of substrate specificity and function in the Escherichia coli hotdog-fold thioesterase paralogs YdiI and YbdB. Biochemistry. 2014 Jul 29;53(29):4775-87. doi: 10.1021/bi500333m. Epub 2014 Jul , 18. PMID:24992697 doi:http://dx.doi.org/10.1021/bi500333m
↑ Wu R, Latham JA, Chen D, Farelli J, Zhao H, Matthews K, Allen KN, Dunaway-Mariano D. Structure and Catalysis in the Escherichia coli Hotdog-fold Thioesterase Paralogs YdiI and YbdB. Biochemistry. 2014 Jul 29;53(29):4788-805. doi: 10.1021/bi500334v. Epub 2014 Jul , 18. PMID:25010423 doi:http://dx.doi.org/10.1021/bi500334v

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4k4b"

@@ Line 1: / Line 1: @@
 ==X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA==
-<StructureSection load='4k4b' size='340' side='right' caption='[[4k4b]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='4k4b' size='340' side='right'caption='[[4k4b]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1sbk|1sbk]], [[4k49|4k49]], [[4k4a|4k4a]], [[4k4c|4k4c]], [[4k4d|4k4d]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [https://pdbe.org/4k4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [https://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/YDII_ECOLI YDII_ECOLI]] Displays esterase activity toward palmitoyl-CoA and pNP-butyrate.
+[https://www.uniprot.org/uniprot/MENI_ECOLI MENI_ECOLI] Catalyzes the hydrolysis of 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA) to 1,4-dihydroxy-2-naphthoate (DHNA). Also shows significant activity toward a wide range of acyl-CoA thioesters, and minimal activity toward benzoyl-holoEntB.<ref>PMID:15808744</ref> <ref>PMID:23564174</ref> <ref>PMID:24992697</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4k4b" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Allen, K N]]
+[[Category: Escherichia coli K-12]]
-[[Category: Dunaway-Mariano, D]]
+[[Category: Large Structures]]
-[[Category: Farelli, J D]]
+[[Category: Allen KN]]
-[[Category: Ru, W]]
+[[Category: Dunaway-Mariano D]]
-[[Category: Hotdog fold]]
+[[Category: Farelli JD]]
-[[Category: Hydrolase]]
+[[Category: Ru W]]
-[[Category: Thioesterase]]

4k4b

From Proteopedia

Current revision

X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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