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4kbm

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{{STRUCTURE_4kbm| PDB=4kbm | SCENE= }}
 
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===Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex===
 
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{{ABSTRACT_PUBMED_24055315}}
 
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==Function==
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==Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex==
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[[http://www.uniprot.org/uniprot/RPOB_MYCTU RPOB_MYCTU]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01321] [[http://www.uniprot.org/uniprot/CARD_MYCTU CARD_MYCTU]] Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for replication and persistence during infection of mice.<ref>PMID:19596241</ref> <ref>PMID:22904282</ref>
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<StructureSection load='4kbm' size='340' side='right'caption='[[4kbm]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kbm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KBM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1146&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kbm OCA], [https://pdbe.org/4kbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kbm RCSB], [https://www.ebi.ac.uk/pdbsum/4kbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kbm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOB_MYCTU RPOB_MYCTU] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01321]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CarD from Mycobacterium tuberculosis (Mtb) is an essential protein shown to be involved in stringent response through downregulation of rRNA and ribosomal protein genes. CarD interacts with the beta-subunit of RNAP and this interaction is vital for Mtb's survival during the persistent infection state. We have determined the crystal structure of CarD in complex with the RNAP beta-subunit beta1 and beta2 domains at 2.1 A resolution. The structure reveals the molecular basis of CarD/RNAP interaction, providing a basis to further our understanding of RNAP regulation by CarD. The structural fold of the CarD N-terminal domain is conserved in RNAP interacting proteins such as TRCF-RID and CdnL, and displays similar interactions to the predicted homology model based on the TRCF/RNAP beta1 structure. Interestingly, the structure of the C-terminal domain, which is required for complete CarD function in vivo, represents a distinct DNA-binding fold.
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==About this Structure==
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Structure of the Mtb CarD/RNAP beta-Lobes Complex Reveals the Molecular Basis of Interaction and Presents a Distinct DNA-Binding Domain for Mtb CarD.,Gulten G, Sacchettini JC Structure. 2013 Sep 17. pii: S0969-2126(13)00306-7. doi:, 10.1016/j.str.2013.08.014. PMID:24055315<ref>PMID:24055315</ref>
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[[4kbm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KBM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024055315</ref><references group="xtra"/><references/>
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</div>
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[[Category: DNA-directed RNA polymerase]]
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<div class="pdbe-citations 4kbm" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Gulten, G.]]
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[[Category: Gulten G]]
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[[Category: Sacchettini, J C.]]
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[[Category: Sacchettini JC]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Card]]
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[[Category: Dna]]
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[[Category: Dna-dependent rna polymerase]]
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[[Category: Protein-protein complex]]
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[[Category: Sigma factor]]
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[[Category: Structural genomic]]
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[[Category: Tb structural genomics consortium]]
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[[Category: Tbsgc]]
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[[Category: Transcription regulator]]
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[[Category: Transferase-transcription complex]]
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[[Category: Tudor]]
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Current revision

Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex

PDB ID 4kbm

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