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4kn9

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{{STRUCTURE_4kn9| PDB=4kn9 | SCENE= }}
 
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===High-resolution structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air===
 
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{{ABSTRACT_PUBMED_10378275}}
 
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==About this Structure==
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==High-resolution structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air==
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[[4kn9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfomicrobium_baculatum Desulfomicrobium baculatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KN9 OCA].
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<StructureSection load='4kn9' size='340' side='right'caption='[[4kn9]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kn9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfomicrobium_baculatum Desulfomicrobium baculatum] and [https://en.wikipedia.org/wiki/Desulfomicrobium_baculatum_DSM_4028 Desulfomicrobium baculatum DSM 4028]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KN9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FCO:CARBONMONOXIDE-(DICYANO)+IRON'>FCO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kn9 OCA], [https://pdbe.org/4kn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kn9 RCSB], [https://www.ebi.ac.uk/pdbsum/4kn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kn9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHSS_DESBA PHSS_DESBA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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This study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.
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==Reference==
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Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.,Volbeda A, Amara P, Iannello M, De Lacey AL, Cavazza C, Fontecilla-Camps JC Chem Commun (Camb). 2013 Jul 11;49(63):7061-3. doi: 10.1039/c3cc43619e. PMID:23811828<ref>PMID:23811828</ref>
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<ref group="xtra">PMID:010378275</ref><references group="xtra"/><references/>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kn9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Desulfomicrobium baculatum]]
[[Category: Desulfomicrobium baculatum]]
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[[Category: Cavazza, C.]]
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[[Category: Desulfomicrobium baculatum DSM 4028]]
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[[Category: Fontecilla-Camps, J C.]]
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[[Category: Large Structures]]
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[[Category: Volbeda, A.]]
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[[Category: Cavazza C]]
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[[Category: H2 cleavage/production]]
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[[Category: Fontecilla-Camps JC]]
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[[Category: Nifese-site]]
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[[Category: Volbeda A]]
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[[Category: O2-resistance]]
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[[Category: Oxidoreductase]]
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Current revision

High-resolution structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air

PDB ID 4kn9

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