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| | ==Structure of the Toll - Spatzle complex, a molecular hub in Drosophila development and innate immunity (glycosylated form)== | | ==Structure of the Toll - Spatzle complex, a molecular hub in Drosophila development and innate immunity (glycosylated form)== |
| - | <StructureSection load='4lxs' size='340' side='right' caption='[[4lxs]], [[Resolution|resolution]] 3.30Å' scene=''> | + | <StructureSection load='4lxs' size='340' side='right'caption='[[4lxs]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lxs]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LXS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lxs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LXS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3e07|3e07]], [[4lxr|4lxr]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tl, CG5490 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), spz, CG6134 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lxs OCA], [https://pdbe.org/4lxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lxs RCSB], [https://www.ebi.ac.uk/pdbsum/4lxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lxs ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lxs OCA], [http://pdbe.org/4lxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lxs RCSB], [http://www.ebi.ac.uk/pdbsum/4lxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lxs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPZ_DROME SPZ_DROME]] Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo. Spz C-106 in the hemolymph controls expression of the antifungal peptide Drosomycin (Drs) by acting as a ligand of Tl and inducing an intracellular signaling pathway.<ref>PMID:8124709</ref> <ref>PMID:11212919</ref> <ref>PMID:8808632</ref> <ref>PMID:12872120</ref> | + | [https://www.uniprot.org/uniprot/TOLL_DROME TOLL_DROME] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Breithaupt, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Parthier, C]] | + | [[Category: Breithaupt C]] |
| - | [[Category: Stelter, M]] | + | [[Category: Parthier C]] |
| - | [[Category: Stubbs, M T]] | + | [[Category: Stelter M]] |
| - | [[Category: Cytokine receptor]] | + | [[Category: Stubbs MT]] |
| - | [[Category: Embryonic development]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immune system-cytokine complex]]
| + | |
| - | [[Category: Innate immunity]]
| + | |
| - | [[Category: Leucine-rich repeat]]
| + | |
| - | [[Category: Receptor-ligand complex]]
| + | |
| - | [[Category: Tlr]]
| + | |
| Structural highlights
Function
TOLL_DROME
Publication Abstract from PubMed
Drosophila Toll receptors are involved in embryonic development and the immune response of adult flies. In both processes, the only known Toll receptor ligand is the human nerve growth factor-like cystine knot protein Spatzle. Here we present the crystal structure of a 1:1 (nonsignaling) complex of the full-length Toll receptor ectodomain (ECD) with the Spatzle cystine knot domain dimer. The ECD is divided into two leucine-rich repeat (LRR) domains, each of which is capped by cysteine-rich domains. Spatzle binds to the concave surface of the membrane-distal LRR domain, in contrast to the flanking ligand interactions observed for mammalian Toll-like receptors, with asymmetric contributions from each Spatzle protomer. The structure allows rationalization of existing genetic and biochemical data and provides a framework for targeting the immune systems of insects of economic importance, as well as a variety of invertebrate disease vectors.
Structure of the Toll-Spatzle complex, a molecular hub in Drosophila development and innate immunity.,Parthier C, Stelter M, Ursel C, Fandrich U, Lilie H, Breithaupt C, Stubbs MT Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6281-6. doi:, 10.1073/pnas.1320678111. Epub 2014 Apr 14. PMID:24733933[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Parthier C, Stelter M, Ursel C, Fandrich U, Lilie H, Breithaupt C, Stubbs MT. Structure of the Toll-Spatzle complex, a molecular hub in Drosophila development and innate immunity. Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6281-6. doi:, 10.1073/pnas.1320678111. Epub 2014 Apr 14. PMID:24733933 doi:http://dx.doi.org/10.1073/pnas.1320678111
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