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| | ==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi== | | ==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi== |
| - | <StructureSection load='4n13' size='340' side='right' caption='[[4n13]], [[Resolution|resolution]] 1.30Å' scene=''> | + | <StructureSection load='4n13' size='340' side='right'caption='[[4n13]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borbu Borbu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N13 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N13 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BB_0215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224326 BORBU])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [http://www.ebi.ac.uk/pdbsum/4n13 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [https://pdbe.org/4n13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [https://www.ebi.ac.uk/pdbsum/4n13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n13 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PSTS_BORBU PSTS_BORBU] Binds inorganic phosphate with a Kd of 1.2 uM (PubMed:24318969). Part of the ABC transporter complex PstSACB involved in phosphate import (Probable).<ref>PMID:24318969</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4n13" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[ABC transporter 3D structures|ABC transporter 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Borbu]] | + | [[Category: Borreliella burgdorferi B31]] |
| - | [[Category: Brautigam, C A]] | + | [[Category: Large Structures]] |
| - | [[Category: Deka, R K]] | + | [[Category: Brautigam CA]] |
| - | [[Category: Norgard, M V]] | + | [[Category: Deka RK]] |
| - | [[Category: Abc transporter]] | + | [[Category: Norgard MV]] |
| - | [[Category: Class ii ligand-binding protein]]
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| - | [[Category: Ligand-binding protein]]
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| - | [[Category: Phosphate-binding protein]]
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| - | [[Category: Transport protein]]
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| Structural highlights
Function
PSTS_BORBU Binds inorganic phosphate with a Kd of 1.2 uM (PubMed:24318969). Part of the ABC transporter complex PstSACB involved in phosphate import (Probable).[1]
Publication Abstract from PubMed
The Lyme disease agent Borrelia burgdorferi, which is transmitted via a tick vector, is dependent on its tick and mammalian hosts for a number of essential nutrients. Like other bacterial diderms, it must transport these biochemicals from the extracellular milieu across two membranes, ultimately to the B. burgdorferi cytoplasm. In the current study, we established that a gene cluster comprising genes bb0215 through bb0218 is cotranscribed and is therefore an operon. Sequence analysis of these proteins suggested that they are the components of an ABC-type transporter responsible for translocating phosphate anions from the B. burgdorferi periplasm to the cytoplasm. Biophysical experiments established that the putative ligand-binding protein of this system, BbPstS (BB0215), binds to phosphate in solution. We determined the high-resolution (1.3 A) crystal structure of the protein in the absence of phosphate, revealing that the protein's fold is similar to other phosphate-binding proteins, and residues that are implicated in phosphate binding in other such proteins are conserved in BbPstS. Taken together, the gene products of bb0215-0218 function as a phosphate transporter for B. burgdorferi.
Sequence, biophysical, and structural analyses of the PstS lipoprotein (BB0215) from Borrelia burgdorferi reveal a likely binding component of an ABC-type phosphate transporter.,Brautigam CA, Ouyang Z, Deka RK, Norgard MV Protein Sci. 2013 Dec 8. doi: 10.1002/pro.2406. PMID:24318969[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Brautigam CA, Ouyang Z, Deka RK, Norgard MV. Sequence, biophysical, and structural analyses of the PstS lipoprotein (BB0215) from Borrelia burgdorferi reveal a likely binding component of an ABC-type phosphate transporter. Protein Sci. 2013 Dec 8. doi: 10.1002/pro.2406. PMID:24318969 doi:http://dx.doi.org/10.1002/pro.2406
- ↑ Brautigam CA, Ouyang Z, Deka RK, Norgard MV. Sequence, biophysical, and structural analyses of the PstS lipoprotein (BB0215) from Borrelia burgdorferi reveal a likely binding component of an ABC-type phosphate transporter. Protein Sci. 2013 Dec 8. doi: 10.1002/pro.2406. PMID:24318969 doi:http://dx.doi.org/10.1002/pro.2406
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