4nea

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: {{STRUCTURE_4nea| PDB=4nea | SCENE= }} ===1.90 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) from Staphylococcus aureus in complex with NAD+ and BME-f...)
Current revision (16:55, 20 September 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4nea| PDB=4nea | SCENE= }}
 
-
===1.90 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) from Staphylococcus aureus in complex with NAD+ and BME-free Cys289===
 
-
==About this Structure==
+
==1.90 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) from Staphylococcus aureus in complex with NAD+ and BME-free Cys289==
-
[[4nea]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_col Staphylococcus aureus subsp. aureus col]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEA OCA].
+
<StructureSection load='4nea' size='340' side='right'caption='[[4nea]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
[[Category: Betaine-aldehyde dehydrogenase]]
+
== Structural highlights ==
-
[[Category: Staphylococcus aureus subsp. aureus col]]
+
<table><tr><td colspan='2'>[[4nea]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NEA FirstGlance]. <br>
-
[[Category: Anderson, W F.]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-
[[Category: Dubrovska, I.]]
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nea OCA], [https://pdbe.org/4nea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nea RCSB], [https://www.ebi.ac.uk/pdbsum/4nea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nea ProSAT]</span></td></tr>
-
[[Category: Halavaty, A S.]]
+
</table>
-
[[Category: Minasov, G.]]
+
== Function ==
-
[[Category: Shuvalova, L.]]
+
[https://www.uniprot.org/uniprot/A0A0H2X0S3_STAAC A0A0H2X0S3_STAAC]
-
[[Category: Winsor, J.]]
+
<div style="background-color:#fffaf0;">
-
[[Category: Center for structural genomics of infectious disease]]
+
== Publication Abstract from PubMed ==
-
[[Category: Csgid]]
+
When exposed to high osmolarity, methicillin-resistant Staphylococcus aureus (MRSA) restores its growth and establishes a new steady state by accumulating the osmoprotectant metabolite betaine. Effective osmoregulation has also been implicated in the acquirement of a profound antibiotic resistance by MRSA. Betaine can be obtained from the bacterial habitat or produced intracellularly from choline via the toxic betaine aldehyde (BA) employing the choline dehydrogenase and betaine aldehyde dehydrogenase (BADH) enzymes. Here, it is shown that the putative betaine aldehyde dehydrogenase SACOL2628 from the early MRSA isolate COL (SaBADH) utilizes betaine aldehyde as the primary substrate and nicotinamide adenine dinucleotide (NAD(+)) as the cofactor. Surface plasmon resonance experiments revealed that the affinity of NAD(+), NADH and BA for SaBADH is affected by temperature, pH and buffer composition. Five crystal structures of the wild type and three structures of the Gly234Ser mutant of SaBADH in the apo and holo forms provide details of the molecular mechanisms of activity and substrate specificity/inhibition of this enzyme.
-
[[Category: Nad+]]
+
 
-
[[Category: National institute of allergy and infectious disease]]
+
Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus.,Halavaty AS, Rich RL, Chen C, Joo JC, Minasov G, Dubrovska I, Winsor JR, Myszka DG, Duban M, Shuvalova L, Yakunin AF, Anderson WF Acta Crystallogr D Biol Crystallogr. 2015 May;71(Pt 5):1159-75. doi:, 10.1107/S1399004715004228. Epub 2015 Apr 25. PMID:25945581<ref>PMID:25945581</ref>
-
[[Category: Niaid]]
+
 
-
[[Category: Oxidoreductase]]
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Rossmann fold]]
+
</div>
 +
<div class="pdbe-citations 4nea" style="background-color:#fffaf0;"></div>
 +
 
 +
==See Also==
 +
*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Staphylococcus aureus subsp. aureus COL]]
 +
[[Category: Anderson WF]]
 +
[[Category: Dubrovska I]]
 +
[[Category: Halavaty AS]]
 +
[[Category: Minasov G]]
 +
[[Category: Shuvalova L]]
 +
[[Category: Winsor J]]

Current revision

1.90 Angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betB) from Staphylococcus aureus in complex with NAD+ and BME-free Cys289

PDB ID 4nea

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4nea"
Personal tools