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4r0o
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of PEGylated plastocyanin at 4.2 A resolution== | |
| + | <StructureSection load='4r0o' size='340' side='right'caption='[[4r0o]], [[Resolution|resolution]] 4.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4r0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_laminosum Phormidium laminosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R0O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=LCY:1-METHYLPYRROLIDINE-2,5-DIONE'>LCY</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0o OCA], [https://pdbe.org/4r0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r0o RCSB], [https://www.ebi.ac.uk/pdbsum/4r0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PLAS_PHOLA PLAS_PHOLA] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PEGylated proteins are a mainstay of the biopharmaceutical industry. Although the use of poly(ethylene glycol) (PEG) to increase particle size, stability and solubility is well-established, questions remain as to the structure of PEG-protein conjugates. Here we report the structural characterization of a model beta-sheet protein (plastocyanin, 11.5 kDa) modified with a single PEG 5,000. An NMR spectroscopy study of the PEGylated conjugate indicated that the protein and PEG behaved as independent domains. A crystal structure revealed an extraordinary double-helical assembly of the conjugate, with the helices arranged orthogonally to yield a highly porous architecture. Electron density was not observed for the PEG chain, which indicates that it was disordered. The volume available per PEG chain in the crystal was within 10% of the calculated random coil volume. Together, these data support a minimal interaction between the protein and the synthetic polymer. Our work provides new possibilities for understanding this important class of protein-polymer hybrids and suggests a novel approach to engineering protein assemblies. | ||
| - | + | Structure of a PEGylated protein reveals a highly porous double-helical assembly.,Cattani G, Vogeley L, Crowley PB Nat Chem. 2015 Oct;7(10):823-8. doi: 10.1038/nchem.2342. Epub 2015 Sep 7. PMID:26391082<ref>PMID:26391082</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4r0o" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Plastocyanin 3D structures|Plastocyanin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Phormidium laminosum]] | ||
| + | [[Category: Cattani G]] | ||
| + | [[Category: Crowley PB]] | ||
| + | [[Category: Vogeley L]] | ||
Current revision
Crystal structure of PEGylated plastocyanin at 4.2 A resolution
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