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4r4u

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Crystal structure of acyl-CoA thioesterase tesB from Yersinia pestis in complex with coenzyme A

Structural highlights

4r4u is a 4 chain structure with sequence from Yersinia pestis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8D151_YERPE

Publication Abstract from PubMed

Acyl-CoA thioesterases catalyse the hydrolysis of the thioester bonds present within a wide range of acyl-CoA substrates, releasing free CoASH and the corresponding fatty-acyl conjugate. The TesB-type thioesterases are members of the TE4 thioesterase family, one of 25 thioesterase enzyme families characterized to date, and contain two fused hotdog domains in both prokaryote and eukaryote homologues. Only two structures have been elucidated within this enzyme family, and much of the current understanding of the TesB thioesterases has been based on the Escherichia coli structure. Yersinia pestis, a highly virulent bacterium, encodes only one TesB-type thioesterase in its genome; here, the structural and functional characterization of this enzyme are reported, revealing unique elements both within the protomer and quaternary arrangements of the hotdog domains which have not been reported previously in any thioesterase family. The quaternary structure, confirmed using a range of structural and biophysical techniques including crystallography, small-angle X-ray scattering, analytical ultracentrifugation and size-exclusion chromatography, exhibits a unique octameric arrangement of hotdog domains. Interestingly, the same biological unit appears to be present in both TesB structures solved to date, and is likely to be a conserved and distinguishing feature of TesB-type thioesterases. Analysis of the Y. pestis TesB thioesterase activity revealed a strong preference for octanoyl-CoA and this is supported by structural analysis of the active site. Overall, the results provide novel insights into the structure of TesB thioesterases which are likely to be conserved and distinguishing features of the TE4 thioesterase family.

Structural and functional characterization of TesB from Yersinia pestis reveals a unique octameric arrangement of hotdog domains.,Swarbrick CM, Perugini MA, Cowieson N, Forwood JK Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):986-95. doi:, 10.1107/S1399004715002527. Epub 2015 Mar 27. PMID:25849407^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Swarbrick CM, Perugini MA, Cowieson N, Forwood JK. Structural and functional characterization of TesB from Yersinia pestis reveals a unique octameric arrangement of hotdog domains. Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):986-95. doi:, 10.1107/S1399004715002527. Epub 2015 Mar 27. PMID:25849407 doi:http://dx.doi.org/10.1107/S1399004715002527

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4r4u"

Categories: Large Structures | Yersinia pestis | Forwood JK | Swarbrick CMD

@@ Line 1: / Line 1: @@
 ==Crystal structure of acyl-CoA thioesterase tesB from Yersinia pestis in complex with coenzyme A==
-<StructureSection load='4r4u' size='340' side='right' caption='[[4r4u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='4r4u' size='340' side='right'caption='[[4r4u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4r4u]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R4U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4r4u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R4U FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qfw|4qfw]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r4u OCA], [http://pdbe.org/4r4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r4u RCSB], [http://www.ebi.ac.uk/pdbsum/4r4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r4u ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r4u OCA], [https://pdbe.org/4r4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r4u RCSB], [https://www.ebi.ac.uk/pdbsum/4r4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r4u ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8D151_YERPE Q8D151_YERPE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 4r4u" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Thioesterase 3D structures|Thioesterase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Forwood, J K]]
+[[Category: Large Structures]]
-[[Category: Swarbrick, C M.D]]
+[[Category: Yersinia pestis]]
-[[Category: 4hbt-like]]
+[[Category: Forwood JK]]
-[[Category: Acyl-coa thioesterase]]
+[[Category: Swarbrick CMD]]
-[[Category: Double-hotdog]]
-[[Category: Hydrolase]]
-[[Category: Thioesterase]]

4r4u

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Current revision

Crystal structure of acyl-CoA thioesterase tesB from Yersinia pestis in complex with coenzyme A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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