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5j3w
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (dark state)== | |
| + | <StructureSection load='5j3w' size='340' side='right'caption='[[5j3w]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5j3w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_KT2440 Pseudomonas putida KT2440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J3W FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j3w OCA], [https://pdbe.org/5j3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j3w RCSB], [https://www.ebi.ac.uk/pdbsum/5j3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j3w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q88E39_PSEPK Q88E39_PSEPK] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Light-Oxygen-Voltage (LOV) domains represent the photo-responsive domains of various blue-light photoreceptor proteins and are widely distributed in plants, algae, fungi, and bacteria. Here, we report the dark-state crystal structure of PpSB1-LOV, a slow-reverting short LOV protein from Pseudomonas putida that is remarkably different from our previously published "fully light-adapted" structure [1]. A direct comparison of the two structures provides insight into the light-activated signaling mechanism. Major structural differences involve a~11A movement of the C terminus in helix Jalpha, ~4A movement of Hbeta-Ibeta loop, disruption of hydrogen bonds in the dimer interface, and a~29 degrees rotation of chain-B relative to chain-A as compared to the light-state dimer. Both crystal structures and solution NMR data are suggestive of the key roles of a conserved glutamine Q116 and the N-cap region consisting of A'alpha-Abeta loop and the A'alpha helix in controlling the light-activated conformational changes. The activation mechanism proposed here for the PpSB1-LOV supports a rotary switch mechanism and provides insights into the signal propagation mechanism in naturally existing and artificial LOV-based, two-component systems and regulators. | ||
| - | + | Signaling States of a Short Blue-Light Photoreceptor Protein PpSB1-LOV Revealed from Crystal Structures and Solution NMR Spectroscopy.,Rollen K, Granzin J, Panwalkar V, Arinkin V, Rani R, Hartmann R, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R J Mol Biol. 2016 Jun 9. pii: S0022-2836(16)30206-6. doi:, 10.1016/j.jmb.2016.05.027. PMID:27291287<ref>PMID:27291287</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5j3w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas putida KT2440]] | ||
| + | [[Category: Batra-Safferling R]] | ||
| + | [[Category: Granzin J]] | ||
Current revision
Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (dark state)
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