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4uam
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4uam is ON HOLD Authors: Carruthers, T.J., Carr, P.D., Jackson, C.J., Otting, G. Description: 1.8 Angstrom crystal structure of IMP-1 metallo-beta-...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==1.8 Angstrom crystal structure of IMP-1 metallo-beta-lactamase with a mixed iron-zinc center in the active site== | |
| + | <StructureSection load='4uam' size='340' side='right'caption='[[4uam]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4uam]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_NCGM2.S1 Pseudomonas aeruginosa NCGM2.S1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UAM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uam OCA], [https://pdbe.org/4uam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uam RCSB], [https://www.ebi.ac.uk/pdbsum/4uam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uam ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Heterodinuclear metalloenzymes are an important class of metalloproteins, but determining the location of the different metal ions can be difficult. Herein we present a new NMR spectroscopy method that uses pseudocontact shifts (PCS) to achieve this without assumptions about the coordinating ligands. The approach is illustrated with the dinuclear [FeZn] complex of IMP-1, which is a prototypical metallo-beta-lactamase (MbetaL) that confers resistance to beta-lactam antibiotics. Results from single-crystal X-ray diffraction were compromised by degradation during crystallization. With [GaZn]-IMP-1 as diamagnetic reference, the PCSs unambiguously identified the iron binding site in fresh samples of [FeZn]-IMP-1, even though the two metal centers are less than 3.8 A apart and the iron is high-spin Fe3+ , which produces only small PCSs. [FeZn]-MbetaLs may be important drug targets, as [FeZn]-IMP-1 is enzymatically active and readily produced in the presence of small amounts of Fe3+ . | ||
| - | + | Iron(III) Located in the Dinuclear Metallo-beta-Lactamase IMP-1 by Pseudocontact Shifts.,Carruthers TJ, Carr PD, Loh CT, Jackson CJ, Otting G Angew Chem Int Ed Engl. 2014 Oct 15. doi: 10.1002/anie.201408693. PMID:25320022<ref>PMID:25320022</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4uam" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa NCGM2 S1]] | ||
| + | [[Category: Carr PD]] | ||
| + | [[Category: Carruthers TJ]] | ||
| + | [[Category: Jackson CJ]] | ||
| + | [[Category: Otting G]] | ||
Current revision
1.8 Angstrom crystal structure of IMP-1 metallo-beta-lactamase with a mixed iron-zinc center in the active site
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