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| | <StructureSection load='4xcc' size='340' side='right'caption='[[4xcc]], [[Resolution|resolution]] 2.81Å' scene=''> | | <StructureSection load='4xcc' size='340' side='right'caption='[[4xcc]], [[Resolution|resolution]] 2.81Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xcc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XCC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xcc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XCC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xaw|4xaw]], [[4xbg|4xbg]], [[4xbe|4xbe]], [[4xc1|4xc1]], [[4xc3|4xc3]], [[4xbp|4xbp]], [[4xce|4xce]], [[4xcf|4xcf]], [[4xcn|4xcn]], [[4xcy|4xcy]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xcc OCA], [http://pdbe.org/4xcc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xcc RCSB], [http://www.ebi.ac.uk/pdbsum/4xcc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xcc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xcc OCA], [https://pdbe.org/4xcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xcc RCSB], [https://www.ebi.ac.uk/pdbsum/4xcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xcc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Irimia, A]] | + | [[Category: Irimia A]] |
| - | [[Category: Stanfield, R L]] | + | [[Category: Stanfield RL]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Wilson IA]] |
| - | [[Category: 4e10 fab anti hiv-1 gp41]]
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| - | [[Category: Immune system]]
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| - | [[Category: Lipid membrane]]
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| - | [[Category: Phosphatidylcholine]]
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| Structural highlights
Publication Abstract from PubMed
Numerous studies of the anti-HIV-1 envelope glycoprotein 41 (gp41) broadly neutralizing antibody 4E10 suggest that 4E10 also interacts with membrane lipids, but the antibody regions contacting lipids and its orientation with respect to the viral membrane are unknown. Vaccine immunogens capable of re-eliciting these membrane proximal external region (MPER)-like antibodies may require a lipid component to be successful. We performed a systematic crystallographic study of lipid binding to 4E10 to identify lipids bound by the antibody and the lipid-interacting regions. We identified phosphatidic acid, phosphatidylglycerol, and glycerol phosphate as specific ligands for 4E10 in the crystal structures. 4E10 used its CDRH1 loop to bind the lipid head groups, while its CDRH3 interacted with the hydrophobic lipid tails. Identification of the lipid binding sites on 4E10 may aid design of immunogens for vaccines that include a lipid component in addition to the MPER on gp41 for generation of broadly neutralizing antibodies.
Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.,Irimia A, Sarkar A, Stanfield RL, Wilson IA Immunity. 2016 Jan 19;44(1):21-31. doi: 10.1016/j.immuni.2015.12.001. Epub 2016, Jan 5. PMID:26777395[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Irimia A, Sarkar A, Stanfield RL, Wilson IA. Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10. Immunity. 2016 Jan 19;44(1):21-31. doi: 10.1016/j.immuni.2015.12.001. Epub 2016, Jan 5. PMID:26777395 doi:http://dx.doi.org/10.1016/j.immuni.2015.12.001
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